Memory and long-term potentiation (LTP) dissociated: Normal spatial memory despite CA1 LTP elimination with <i>Kv1.4</i> antisense
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- Noam Meiri
- Laboratory of Adaptive Systems, National Institutes of Health, Bethesda, MD 20892
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- Miao-Kun Sun
- Laboratory of Adaptive Systems, National Institutes of Health, Bethesda, MD 20892
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- Zachary Segal
- Laboratory of Adaptive Systems, National Institutes of Health, Bethesda, MD 20892
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- Daniel L. Alkon
- Laboratory of Adaptive Systems, National Institutes of Health, Bethesda, MD 20892
説明
<jats:p> Long-term potentiation (LTP) in the hippocampal slice preparation has been proposed as an <jats:italic>in vitro</jats:italic> model for long-term memory. However, correlation of LTP with memory in living animals has been difficult to demonstrate. Furthermore, in the last few years evidence has accumulated that dissociate the two. Because potassium channels might determine the weight of synapses in networks, we studied the role of Kv1.4, a presynaptic A-type voltage-dependent K <jats:sup>+</jats:sup> channel, in both memory and LTP. Reverse transcription–PCR and Western blot analysis with specific antibodies showed that antisense oligodeoxyribonucleotide to Kv1.4 microinjected intraventricularly into rat brains obstructed hippocampal Kv1.4 mRNA, “knocking down” the protein in the hippocampus. This antisense knockdown had no effect on rat spatial maze learning, memory, or exploratory behavior, but eliminated both early- and late-phase LTP and reduced paired-pulse facilitation (a presynaptic effect) in CA1 pyramidal neurons without affecting dentate gyrus LTP. This presynaptic Kv1.4 knockdown together with previous postsynaptic Kv1.1 knockdown demonstrates that CA1 LTP is neither necessary nor sufficient for rat spatial memory. </jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 95 (25), 15037-15042, 1998-12-08
Proceedings of the National Academy of Sciences
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詳細情報 詳細情報について
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- CRID
- 1361981468721576320
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- ISSN
- 10916490
- 00278424
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- データソース種別
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- Crossref