Quantitative analysis of hepatitis C virus-specific CD8<sup>+</sup>T cells in peripheral blood and liver using peptide-MHC tetramers

DOI Web Site 被引用文献3件
  • Xiao-Song He
    Departments of Medicine and Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305; Liver Diseases Section, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892; Howard Hughes Medical Institute; and Department of Medicine, University of California, San Francisco, CA 94121
  • Barbara Rehermann
    Departments of Medicine and Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305; Liver Diseases Section, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892; Howard Hughes Medical Institute; and Department of Medicine, University of California, San Francisco, CA 94121
  • F. Xavier López-Labrador
    Departments of Medicine and Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305; Liver Diseases Section, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892; Howard Hughes Medical Institute; and Department of Medicine, University of California, San Francisco, CA 94121
  • Judie Boisvert
    Departments of Medicine and Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305; Liver Diseases Section, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892; Howard Hughes Medical Institute; and Department of Medicine, University of California, San Francisco, CA 94121
  • Ramsey Cheung
    Departments of Medicine and Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305; Liver Diseases Section, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892; Howard Hughes Medical Institute; and Department of Medicine, University of California, San Francisco, CA 94121
  • John Mumm
    Departments of Medicine and Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305; Liver Diseases Section, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892; Howard Hughes Medical Institute; and Department of Medicine, University of California, San Francisco, CA 94121
  • Heiner Wedemeyer
    Departments of Medicine and Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305; Liver Diseases Section, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892; Howard Hughes Medical Institute; and Department of Medicine, University of California, San Francisco, CA 94121
  • Marina Berenguer
    Departments of Medicine and Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305; Liver Diseases Section, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892; Howard Hughes Medical Institute; and Department of Medicine, University of California, San Francisco, CA 94121
  • Teresa L. Wright
    Departments of Medicine and Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305; Liver Diseases Section, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892; Howard Hughes Medical Institute; and Department of Medicine, University of California, San Francisco, CA 94121
  • Mark M. Davis
    Departments of Medicine and Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305; Liver Diseases Section, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892; Howard Hughes Medical Institute; and Department of Medicine, University of California, San Francisco, CA 94121
  • Harry B. Greenberg
    Departments of Medicine and Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305; Liver Diseases Section, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892; Howard Hughes Medical Institute; and Department of Medicine, University of California, San Francisco, CA 94121

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<jats:p>It is believed that the hepatitis C virus (HCV)-specific CD8<jats:sup>+</jats:sup>cytotoxic T lymphocytes (CTLs) play a role in the development of liver cell injury and in the clearance of the virus. To develop a direct binding assay for HCV-specific CTLs, we generated two peptide-MHC tetramers by using the recombinant HLA A2.1 molecule and A2-restricted T cell epitopes of the HCV NS3 protein. With these reagents we are able to detect specific CD8<jats:sup>+</jats:sup>cells in the blood of 15 of 20 HLA-A2<jats:sup>+</jats:sup>, HCV-infected patients, at a frequency ranging from 0.01% to 1.2% of peripheral CD8<jats:sup>+</jats:sup>T cells. Phenotypic analysis of these specific cells indicated that there is a significant variation in the expression of the CD45 isoforms and CD27 in different patients. A 6-hour incubation of one patient’s blood with NS3 peptides resulted in the activation of the epitope-specific CD8<jats:sup>+</jats:sup>cells, as indicated by their expression of CD69 and IFN-γ. We also detected NS3-specific CD8<jats:sup>+</jats:sup>T cells in the intrahepatic lymphocyte population isolated from liver biopsies of two HCV-infected patients. The frequency of these specific CD8<jats:sup>+</jats:sup>cells in the liver was 1–2%, at least 30-fold higher than in the peripheral blood. All of the intrahepatic NS3-specific CD8<jats:sup>+</jats:sup>T cells were CD69<jats:sup>+</jats:sup>, suggesting that they were activated CTLs. Direct quantitation and characterization of HCV-specific CTLs should extend our understanding of the immunopathogenesis and the mechanism of clearance or persistence of HCV.</jats:p>

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