Marinilactibacillus piezotolerans sp. nov., a novel marine lactic acid bacterium isolated from deep sub-seafloor sediment of the Nankai Trough

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  • Laurent Toffin
    UMR 6539, Centre National de la Recherche Scientifique et Université de Bretagne Occidentale, Technopôle Brest-Iroise, Place Nicolas Copernic, F-29280 Plouzané, France
  • Klaus Zink
    GeoForschungsZentrumPotsdam (GFZ), Telegrafenberg, D-14473 Potsdam, Germany
  • Chiaki Kato
    Extremobiosphere Research Centre, Japan Agency for Marine–Earth Science and Technology (JAMSTEC), 2–15 Natsushima-cho, Yokosuka 237-0061, Japan
  • Patricia Pignet
    Laboratoire de Microbiologie et de Biotechnologie des Extrêmophiles, Département de Valorisation des Produits, IFREMER, Centre de Brest, BP 70, F-29280 Plouzané, France
  • Adeline Bidault
    UMR 6539, Centre National de la Recherche Scientifique et Université de Bretagne Occidentale, Technopôle Brest-Iroise, Place Nicolas Copernic, F-29280 Plouzané, France
  • Nadège Bienvenu
    UMR 6539, Centre National de la Recherche Scientifique et Université de Bretagne Occidentale, Technopôle Brest-Iroise, Place Nicolas Copernic, F-29280 Plouzané, France
  • Jean-Louis Birrien
    UMR 6539, Centre National de la Recherche Scientifique et Université de Bretagne Occidentale, Technopôle Brest-Iroise, Place Nicolas Copernic, F-29280 Plouzané, France
  • Daniel Prieur
    UMR 6539, Centre National de la Recherche Scientifique et Université de Bretagne Occidentale, Technopôle Brest-Iroise, Place Nicolas Copernic, F-29280 Plouzané, France
  • 4.3 Organic Geochemistry, 4.0 Chemistry and Material Cycles, Departments, GFZ Publication Database, Deutsches GeoForschungsZentrum

Description

<jats:p>A piezotolerant, mesophilic, marine lactic acid bacterium (strain LT20<jats:sup>T</jats:sup>) was isolated from a deep sub-seafloor sediment core collected at Nankai Trough, off the coast of Japan. Cells were Gram-positive, rod-shaped, non-sporulating and non-motile. The NaCl concentration range for growth was 0–120 g l<jats:sup>−1</jats:sup>, with the optimum at 10–20 g l<jats:sup>−1</jats:sup>. The temperature range for growth at pH 7·0 was 4–50 °C, with the optimum at 37–40 °C. The optimum pH for growth was 7·0–8·0. The optimum pressure for growth was 0·1 MPa with tolerance up to 30 MPa. The main cellular phospholipids were phosphatidylglycerols (25 %), diphosphatidylglycerols (34 %) and a group of compounds tentatively identified as ammonium-containing phosphatidylserines (32 %); phosphatidylethanolamines (9 %) were minor components. The fatty acid composition was dominated by side chains of 16 : 0, 14 : 0 and 16 : 1. The G+C content of the genomic DNA was 42 mol%. On the basis of 16S rRNA gene sequence analysis and the secondary structure of the V6 region, this organism was found to belong to the genus<jats:italic>Marinilactibacillus</jats:italic>and was closely related to<jats:italic>Marinilactibacillus psychrotolerans</jats:italic>M13-2<jats:sup>T</jats:sup>(99 %),<jats:italic>Marinilactibacillus</jats:italic>sp. strain MJYP.25.24 (99 %) and<jats:italic>Alkalibacterium olivapovliticus</jats:italic>strain ww2-SN4C (97 %). Despite the high similarity between their 16S rRNA gene sequences (99 %), the DNA–DNA hybridization levels were less than 20 %. On the basis of physiological and genetic characteristics, it is proposed that this organism be classified as a novel species,<jats:italic>Marinilactibacillus piezotolerans</jats:italic>sp. nov. The type strain is LT20<jats:sup>T</jats:sup>(=DSM 16108<jats:sup>T</jats:sup>=JCM 12337<jats:sup>T</jats:sup>).</jats:p>

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