Stem Cell Factor in Mast Cells and Increased Mast Cell Density in Idiopathic and Ischemic Cardiomyopathy

DOI Web Site 6 Citations
  • Vincenzo Patella
    From the Division of Clinical Immunology and Allergy (V.P., I.M., G.M.), University of Naples Federico II, School of Medicine, Napoli, Italy; Department of Pathology (E.A., L.V.), University of Pavia, School of Medicine, Pavia, Italy; and Department of Anesthesiology (B.L.-S., M.A.), Deutsches Herzzentrum Berlin, Berlin, Germany.
  • Isabella Marinò
    From the Division of Clinical Immunology and Allergy (V.P., I.M., G.M.), University of Naples Federico II, School of Medicine, Napoli, Italy; Department of Pathology (E.A., L.V.), University of Pavia, School of Medicine, Pavia, Italy; and Department of Anesthesiology (B.L.-S., M.A.), Deutsches Herzzentrum Berlin, Berlin, Germany.
  • Eloisa Arbustini
    From the Division of Clinical Immunology and Allergy (V.P., I.M., G.M.), University of Naples Federico II, School of Medicine, Napoli, Italy; Department of Pathology (E.A., L.V.), University of Pavia, School of Medicine, Pavia, Italy; and Department of Anesthesiology (B.L.-S., M.A.), Deutsches Herzzentrum Berlin, Berlin, Germany.
  • Bärbel Lamparter-Schummert
    From the Division of Clinical Immunology and Allergy (V.P., I.M., G.M.), University of Naples Federico II, School of Medicine, Napoli, Italy; Department of Pathology (E.A., L.V.), University of Pavia, School of Medicine, Pavia, Italy; and Department of Anesthesiology (B.L.-S., M.A.), Deutsches Herzzentrum Berlin, Berlin, Germany.
  • Laura Verga
    From the Division of Clinical Immunology and Allergy (V.P., I.M., G.M.), University of Naples Federico II, School of Medicine, Napoli, Italy; Department of Pathology (E.A., L.V.), University of Pavia, School of Medicine, Pavia, Italy; and Department of Anesthesiology (B.L.-S., M.A.), Deutsches Herzzentrum Berlin, Berlin, Germany.
  • Monika Adt
    From the Division of Clinical Immunology and Allergy (V.P., I.M., G.M.), University of Naples Federico II, School of Medicine, Napoli, Italy; Department of Pathology (E.A., L.V.), University of Pavia, School of Medicine, Pavia, Italy; and Department of Anesthesiology (B.L.-S., M.A.), Deutsches Herzzentrum Berlin, Berlin, Germany.
  • Gianni Marone
    From the Division of Clinical Immunology and Allergy (V.P., I.M., G.M.), University of Naples Federico II, School of Medicine, Napoli, Italy; Department of Pathology (E.A., L.V.), University of Pavia, School of Medicine, Pavia, Italy; and Department of Anesthesiology (B.L.-S., M.A.), Deutsches Herzzentrum Berlin, Berlin, Germany.

Abstract

<jats:p> <jats:italic>Background</jats:italic> —We compared cardiac mast cell (HHMC) density and the immunological and nonimmunological release of mediators from mast cells isolated from heart tissue of patients with idiopathic dilated (DCM) (n=24) and ischemic cardiomyopathy (ICM) (n=10) undergoing heart transplantation and from control subjects (n=10) without cardiovascular disease. </jats:p> <jats:p> <jats:italic>Methods and Results</jats:italic> —HHMC density in DCM (18.4±1.6 cells/mm <jats:sup>2</jats:sup> ) and ICM (18.4±1.5 cells/mm <jats:sup>2</jats:sup> ) was higher than that in control hearts (5.3±0.7 cells/mm <jats:sup>2</jats:sup> ; <jats:italic>P</jats:italic> <.01). The histamine and tryptase contents of DCM and ICM hearts were higher than those of control hearts. The histamine content of the hearts was correlated with mast cell density ( <jats:italic>r</jats:italic> <jats:sub>s</jats:sub> =.91; <jats:italic>P</jats:italic> <.001). Protein A/gold staining of heart tissue revealed stem cell factor (SCF), the principal growth, differentiating, and activating factor of human mast cells, in HHMC secretory granules. Histamine release from cardiac mast cells caused by immunological (anti-IgE and rhSCF) and nonimmunological stimuli (Ca <jats:sup>2+</jats:sup> ionophore A23187) was higher in patients with DCM and ICM compared with control subjects. Immunological activation of HHMC induced a significantly greater release of tryptase and LTC <jats:sub>4</jats:sub> in patients with DCM and ICM compared with control subjects. </jats:p> <jats:p> <jats:italic>Conclusions</jats:italic> —Histamine and tryptase content and mast cell density are higher in failing hearts than in control hearts. SCF, present in secretory granules of HHMC, might represent an autocrine factor sustaining mast cell hyperplasia in heart tissue in these patients. The increased local release of fibrogenic factors (eg, histamine, tryptase, and leukotriene C <jats:sub>4</jats:sub> ) might contribute to collagen accumulation in the hearts of patients with cardiomyopathy. </jats:p>

Journal

  • Circulation

    Circulation 97 (10), 971-978, 1998-03-17

    Ovid Technologies (Wolters Kluwer Health)

Citations (6)*help

See more

Details 詳細情報について

Report a problem

Back to top