Adherence of Vibrio cholerae to cultured differentiated human intestinal cells: an in vitro colonization model
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- J A Bénitez
- Centro Nacional de Investigaciones Científicas, Havana, Cuba.
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- R G Spelbrink
- Centro Nacional de Investigaciones Científicas, Havana, Cuba.
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- A Silva
- Centro Nacional de Investigaciones Científicas, Havana, Cuba.
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- T E Phillips
- Centro Nacional de Investigaciones Científicas, Havana, Cuba.
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- C M Stanley
- Centro Nacional de Investigaciones Científicas, Havana, Cuba.
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- M Boesman-Finkelstein
- Centro Nacional de Investigaciones Científicas, Havana, Cuba.
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- R A Finkelstein
- Centro Nacional de Investigaciones Científicas, Havana, Cuba.
説明
<jats:p>Choleragenic vibrios adhered to and multiplied on monolayers of the highly differentiated mucin-secreting cell line HT29-18N2. Their adherence followed first-order kinetics, was dependent on the concentration of vibrios, and was partially inhibited by lipopolysaccharide. Comparison of genetically modified vibrios showed that flagella, an active toxR gene, and the virulence cassette were not essential for initial binding. Inactivation of the hemagglutinin/protease increased binding. This highly differentiated human intestinal cell line provides a versatile new approach for studying major events occurring during intestinal colonization: adherence, multiplication, and detachment.</jats:p>
収録刊行物
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- Infection and Immunity
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Infection and Immunity 65 (8), 3474-3477, 1997-08
American Society for Microbiology