Dynamics of Centromeres during Metaphase–Anaphase Transition in Fission Yeast: Dis1 Is Implicated in Force Balance in Metaphase Bipolar Spindle

  • Kentaro Nabeshima
    CREST Research Project, Department of Biophysics, Graduate School of Science, Kyoto University, Kyoto 606, Japan;
  • Takashi Nakagawa
    CREST Research Project, Department of Biophysics, Graduate School of Science, Kyoto University, Kyoto 606, Japan;
  • Aaron F. Straight
    Department of Physiology, University of California San Francisco, San Francisco, California 94143-0448; and
  • Andrew Murray
    Department of Physiology, University of California San Francisco, San Francisco, California 94143-0448; and
  • Yuji Chikashige
    Kansai Advanced Research Center, Communications Research Laboratory, Kobe 651-24, Japan
  • Yukiko M. Yamashita
    CREST Research Project, Department of Biophysics, Graduate School of Science, Kyoto University, Kyoto 606, Japan;
  • Yasushi Hiraoka
    Kansai Advanced Research Center, Communications Research Laboratory, Kobe 651-24, Japan
  • Mitsuhiro Yanagida
    CREST Research Project, Department of Biophysics, Graduate School of Science, Kyoto University, Kyoto 606, Japan;

抄録

<jats:p>In higher eukaryotic cells, the spindle forms along with chromosome condensation in mitotic prophase. In metaphase, chromosomes are aligned on the spindle with sister kinetochores facing toward the opposite poles. In anaphase A, sister chromatids separate from each other without spindle extension, whereas spindle elongation takes place during anaphase B. We have critically examined whether such mitotic stages also occur in a lower eukaryote, Schizosaccharomyces pombe. Using the green fluorescent protein tagging technique, early mitotic to late anaphase events were observed in living fission yeast cells. S. pombe has three phases in spindle dynamics, spindle formation (phase 1), constant spindle length (phase 2), and spindle extension (phase 3). Sister centromere separation (anaphase A) rapidly occurred at the end of phase 2. The centromere showed dynamic movements throughout phase 2 as it moved back and forth and was transiently split in two before its separation, suggesting that the centromere was positioned in a bioriented manner toward the poles at metaphase. Microtubule-associating Dis1 was required for the occurrence of constant spindle length and centromere movement in phase 2. Normal transition from phase 2 to 3 needed DNA topoisomerase II and Cut1 but not Cut14. The duration of each phase was highly dependent on temperature.</jats:p>

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詳細情報 詳細情報について

  • CRID
    1361981469581542016
  • DOI
    10.1091/mbc.9.11.3211
  • ISSN
    19394586
    10591524
  • データソース種別
    • Crossref

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