Chloroplast Transformation in <i>Chlamydomonas</i> with High Velocity Microprojectiles
-
- John E. Boynton
- Department of Botany, Duke University, Durham, NC 27706.
-
- Nicholas W. Gillham
- Department of Zoology, Duke University, Durham, NC 27706.
-
- Elizabeth H. Harris
- Department of Botany, Duke University, Durham, NC 27706.
-
- Jonathan P. Hosler
- Department of Botany, Duke University, Durham, NC 27706.
-
- Anita M. Johnson
- Department of Botany, Duke University, Durham, NC 27706.
-
- Allan R. Jones
- Department of Botany, Duke University, Durham, NC 27706.
-
- Barbara L. Randolph-Anderson
- Department of Botany, Duke University, Durham, NC 27706.
-
- Dominique Robertson
- Department of Zoology, Duke University, Durham, NC 27706.
-
- Ted M. Klein
- New York State Agricultural Experiment Station, Cornell University, Geneva, NY 14456.
-
- Katherine B. Shark
- New York State Agricultural Experiment Station, Cornell University, Geneva, NY 14456.
-
- John C. Sanford
- New York State Agricultural Experiment Station, Cornell University, Geneva, NY 14456.
書誌事項
- 公開日
- 1988-06-10
- DOI
-
- 10.1126/science.2897716
- 公開者
- American Association for the Advancement of Science (AAAS)
この論文をさがす
説明
<jats:p> Bombardment of three mutants of the chloroplast <jats:italic>atpB</jats:italic> gene of <jats:italic>Chlamydomonas reinhardtii</jats:italic> with high-velocity tungsten microprojectiles that were coated with cloned chloroplast DNA carrying the wild-type gene permanently restored the photosynthetic capacity of the algae. In most transformants of one of the mutants, a fragment with a 2.5-kilobase deletion was restored to normal size by a homologous replacement event; in about 25 percent of the transformants the restored restriction fragment was 50 to 100 base pairs smaller or larger than that of wild type. About one-fourth of the transformants of this mutant contained unintegrated donor plasmid when first examined. This plasmid persisted in four different transformants after 65 cell generations of continuous liquid culture but was lost from all transformants maintained on plates of selective medium. The restored wild-type <jats:italic>atpB</jats:italic> gene remains in all transformants as an integral part of the chloroplast genome and is expressed and inherited normally. </jats:p>
収録刊行物
-
- Science
-
Science 240 (4858), 1534-1538, 1988-06-10
American Association for the Advancement of Science (AAAS)
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1361981469976141952
-
- NII論文ID
- 80004113443
-
- ISSN
- 10959203
- 00368075
- http://id.crossref.org/issn/00368075
-
- データソース種別
-
- Crossref
- CiNii Articles
