Reciprocal Silencing, Transcriptional Bias and Functional Divergence of Homeologs in Polyploid Cotton (Gossypium)
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- Bhupendra Chaudhary
- Department of Ecology , Evolution, and Organismal Biology, Iowa State University, Ames, Iowa 50011
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- Lex Flagel
- Department of Ecology , Evolution, and Organismal Biology, Iowa State University, Ames, Iowa 50011
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- Robert M Stupar
- Departments of Agronomy and Plant Genetics and
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- Joshua A Udall
- Department of Plant and Wildlife Sciences , Brigham Young University, Provo, Utah 84602
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- Neetu Verma
- Department of Ecology , Evolution, and Organismal Biology, Iowa State University, Ames, Iowa 50011
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- Nathan M Springer
- Plant Biology , University of Minnesota, St. Paul, Minnesota 55108 and
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- Jonathan F Wendel
- Department of Ecology , Evolution, and Organismal Biology, Iowa State University, Ames, Iowa 50011
Description
<jats:title>Abstract</jats:title><jats:p>Polyploidy is an important force in the evolution of flowering plants. Genomic merger and doubling induce an extensive array of genomic effects, including immediate and long-term alterations in the expression of duplicate genes (“homeologs”). Here we employed a novel high-resolution, genome-specific, mass-spectrometry technology and a well-established phylogenetic framework to investigate relative expression levels of each homeolog for 63 gene pairs in 24 tissues in naturally occurring allopolyploid cotton (Gossypium L.), a synthetic allopolyploid of the same genomic composition, and models of the diploid progenitor species. Results from a total of 2177 successful expression assays permitted us to determine the extent of expression evolution accompanying genomic merger of divergent diploid parents, genome doubling, and genomic coevolution in a common nucleus subsequent to polyploid formation. We demonstrate that 40% of homeologs are transcriptionally biased in at least one stage of cotton development, that genome merger per se has a large effect on relative expression of homeologs, and that the majority of these alterations are caused by cis-regulatory divergence between the diploid progenitors. We describe the scope of transcriptional subfunctionalization and 15 cases of probable neofunctionalization among 8 tissues. To our knowledge, this study represents the first characterization of transcriptional neofunctionalization in an allopolyploid. These results provide a novel temporal perspective on expression evolution of duplicate genomes and add to our understanding of the importance of polyploidy in plants.</jats:p>
Journal
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- Genetics
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Genetics 182 (2), 503-517, 2009-06-01
Oxford University Press (OUP)
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Details 詳細情報について
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- CRID
- 1361981470006131968
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- ISSN
- 19432631
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- Data Source
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- Crossref