Efficacy of ozone on survival and permeability of oral microorganisms
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説明
<jats:p>In the present study, we examined the effect of ozonated water on oral microorganisms and dental plaque. Almost no microorganisms were detected after being treated with ozonated water (4 mg/l) for 10 s. To estimate the ozonated water‐treated <jats:italic>Streptococcus mutans,</jats:italic> bacterial cells were stained with LIVE/DEAD<jats:sup>®</jats:sup><jats:italic>Bac</jats:italic>Light<jats:sup>TM</jats:sup> Bacterial Viability Kit. Fluorescence microscopic analysis revealed that <jats:italic>S. mutans</jats:italic> cells were killed instantaneously in ozonated water. Some breakage of ozonated water‐treated <jats:italic>S. mutans</jats:italic> was found by electron microscopy. When the experimental dental plaque was exposed to ozonated water, the number of viable <jats:italic>S. mutans</jats:italic> remarkably decreased. Ozonated water strongly inhibited the accumulation of experimental dental plaque <jats:italic>in vitro</jats:italic>. After the dental plaque samples from human subjects were exposed to ozonated water <jats:italic>in vitro</jats:italic>, almost no viable bacterial cells were detected. These results suggest that ozonated water should be useful in reducing the infections caused by oral microorganisms in dental plaque.</jats:p>
収録刊行物
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- Oral Microbiology and Immunology
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Oral Microbiology and Immunology 19 (4), 240-246, 2004-06-22
Wiley