Diphtheria toxin receptor–mediated conditional and targeted cell ablation in transgenic mice

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書誌事項

公開日
2001-08
権利情報
  • http://www.springer.com/tdm
  • http://www.springer.com/tdm
DOI
  • 10.1038/90795
公開者
Springer Science and Business Media LLC

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説明

Specific cell ablation is a useful method for analyzing the in vivo function of cells. We have developed a simple and sensitive method for conditional cell ablation in transgenic mice, called "toxin receptor-mediated cell knockout." We expressed the diphtheria toxin (DT) receptor in transgenic mice using a hepatocyte-specific promoter and found that injection of DT caused fulminant hepatitis. Three independently established transgenic lines demonstrated a good correlation between the sensitivity of hepatocytes to DT and the expression level of the DT receptors. Moreover, the degree of hepatocyte damage was easily controlled over a wide range of doses of injected DT without any obvious abnormalities in other cells or tissues. This system is useful for generating mouse models of disease and for studying the recovery or regeneration of tissues from cell damage or loss. As DT is a potent inhibitor of protein synthesis in both growing and non-growing cells, the method is applicable to a wide range of cells and tissues in mice or in other DT-insensitive animals.

収録刊行物

  • Nature Biotechnology

    Nature Biotechnology 19 (8), 746-750, 2001-08

    Springer Science and Business Media LLC

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