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- G.P. Jevon
- Department of Pathology, Children's and Woman's Health Centre of British Columbia and University of British Columbia, 4480 Oak Street, Vancouver, BC, Canada V6H 3V4
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- J.E. Dimmick
- Department of Pathology, Children's and Woman's Health Centre of British Columbia and University of British Columbia, 4480 Oak Street, Vancouver, BC, Canada V6H 3V4
書誌事項
- 公開日
- 1999-01
- 権利情報
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- https://journals.sagepub.com/page/policies/text-and-data-mining-license
- DOI
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- 10.1007/s100249900083
- 公開者
- SAGE Publications
この論文をさがす
説明
<jats:p> The cause of extrahepatic biliary atresia (EHBA) is undetermined in most instances, but an infectious agent is widely suspected. Cytomegalovirus (CMV) infection has been associated with intrahepatic bile duct destruction and paucity raising the question of its role in EHBA. We identified 12 children in the past 5 years with biliary atresia and examined the bile duct biopsy. These showed acute/chronic inflammation and epithelial degeneration. CMV inclusions were not identified. We used in situ hybridization and the polymerase chain reaction (PCR) for CMV-DNA on formalin-fixed, paraffin-embedded tissue. All samples showed the presence of amplifiable DNA using β-globin primers. No biopsy tissue showed CMV DNA using specific probes and primers. The absence of demonstrable CMV DNA by in situ hybridization and PCR in EHBA biopsies implies that it is unlikely that this virus has any major role in the pathogenesis of this condition. </jats:p>
収録刊行物
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- Pediatric and Developmental Pathology
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Pediatric and Developmental Pathology 2 (1), 11-14, 1999-01
SAGE Publications