Stability issues of RT‐PCR testing of SARS‐CoV‐2 for hospitalized patients clinically diagnosed with COVID‐19

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  • Yafang Li
    Department of Intensive Care Unit, The Sixth Affiliated Hospital Sun Yat‐sen University Guangzhou China
  • Lin Yao
    Department of Operation and Management The Sixth Affiliated Hospital, Sun Yat‐sen University Guangzhou China
  • Jiawei Li
    Natural Language Processing Group iFLYTEK Research South China Guangzhou China
  • Lei Chen
    Department of Intensive Care Unit, The Sixth Affiliated Hospital Sun Yat‐sen University Guangzhou China
  • Yiyan Song
    Department of Clinical Medicine Zhongshan School of Medicine Guangzhou China
  • Zhifang Cai
    Department of Respiratory Hankou Hospital of Wuhan Wuhan China
  • Chunhua Yang
    Department of Intensive Care Unit, The Sixth Affiliated Hospital Sun Yat‐sen University Guangzhou China

Description

<jats:title>Abstract</jats:title><jats:p>In this study, we collected a total of 610 hospitalized patients from Wuhan between February 2, 2020, and February 17, 2020. We reported a potentially high false negative rate of real‐time reverse‐transcriptase polymerase chain reaction (RT‐PCR) testing for SARS‐CoV‐2 in the 610 hospitalized patients clinically diagnosed with COVID‐19 during the 2019 outbreak. We also found that the RT‐PCR results from several tests at different points were variable from the same patients during the course of diagnosis and treatment of these patients. Our results indicate that in addition to the emphasis on RT‐PCR testing, clinical indicators such as computed tomography images should also be used not only for diagnosis and treatment but also for isolation, recovery/discharge, and transferring for hospitalized patients clinically diagnosed with COVID‐19 during the current epidemic. These results suggested the urgent needs for the standard of procedures of sampling from different anatomic sites, sample transportation, optimization of RT‐PCR, serology diagnosis/screening for SARS‐CoV‐2 infection, and distinct diagnosis from other respiratory diseases such as fluenza infections as well.</jats:p>

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