Quantitative 3D imaging of whole, unstained cells by using X-ray diffraction microscopy
-
- Huaidong Jiang
- Department of Physics and Astronomy and California NanoSystems Institute, University of California, Los Angeles, CA 90095;
-
- Changyong Song
- RIKEN SPring-8 Center, 1-1-1, Kouto, Sayo, Hyogo 679-5148, Japan;
-
- Chien-Chun Chen
- Department of Physics and Astronomy and California NanoSystems Institute, University of California, Los Angeles, CA 90095;
-
- Rui Xu
- Department of Physics and Astronomy and California NanoSystems Institute, University of California, Los Angeles, CA 90095;
-
- Kevin S. Raines
- Department of Physics and Astronomy and California NanoSystems Institute, University of California, Los Angeles, CA 90095;
-
- Benjamin P. Fahimian
- Department of Physics and Astronomy and California NanoSystems Institute, University of California, Los Angeles, CA 90095;
-
- Chien-Hung Lu
- Institute of Physics, Academia Sinica, Nankang, Taipei 11529, Taiwan; and
-
- Ting-Kuo Lee
- Institute of Physics, Academia Sinica, Nankang, Taipei 11529, Taiwan; and
-
- Akio Nakashima
- Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA 90095
-
- Jun Urano
- Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA 90095
-
- Tetsuya Ishikawa
- RIKEN SPring-8 Center, 1-1-1, Kouto, Sayo, Hyogo 679-5148, Japan;
-
- Fuyuhiko Tamanoi
- Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA 90095
-
- Jianwei Miao
- Department of Physics and Astronomy and California NanoSystems Institute, University of California, Los Angeles, CA 90095;
書誌事項
- 公開日
- 2010-06-04
- DOI
-
- 10.1073/pnas.1000156107
- 公開者
- Proceedings of the National Academy of Sciences
この論文をさがす
説明
<jats:p>Microscopy has greatly advanced our understanding of biology. Although significant progress has recently been made in optical microscopy to break the diffraction-limit barrier, reliance of such techniques on fluorescent labeling technologies prohibits quantitative 3D imaging of the entire contents of cells. Cryoelectron microscopy can image pleomorphic structures at a resolution of 3–5 nm, but is only applicable to thin or sectioned specimens. Here, we report quantitative 3D imaging of a whole, unstained cell at a resolution of 50–60 nm by X-ray diffraction microscopy. We identified the 3D morphology and structure of cellular organelles including cell wall, vacuole, endoplasmic reticulum, mitochondria, granules, nucleus, and nucleolus inside a yeast spore cell. Furthermore, we observed a 3D structure protruding from the reconstructed yeast spore, suggesting the spore germination process. Using cryogenic technologies, a 3D resolution of 5–10 nm should be achievable by X-ray diffraction microscopy. This work hence paves a way for quantitative 3D imaging of a wide range of biological specimens at nanometer-scale resolutions that are too thick for electron microscopy.</jats:p>
収録刊行物
-
- Proceedings of the National Academy of Sciences
-
Proceedings of the National Academy of Sciences 107 (25), 11234-11239, 2010-06-04
Proceedings of the National Academy of Sciences