cDNA cloning, expression and characterization of human prostaglandin F synthase<sup>1</sup>

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書誌事項

公開日
1999-11-30
権利情報
  • http://onlinelibrary.wiley.com/termsAndConditions#vor
DOI
  • 10.1016/s0014-5793(99)01551-3
公開者
Wiley

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説明

<jats:p>A cDNA clone of prostaglandin F synthase (PGFS) was isolated from human lung by using cDNA of bovine lung‐type PGFS as a probe and its protein expressed in <jats:italic>Escherichia coli</jats:italic> was purified to apparent homogeneity. The human PGFS catalyzed the reduction of prostaglandin (PG) D<jats:sub>2</jats:sub>, PGH<jats:sub>2</jats:sub> and phenanthrenequinone (PQ), and the oxidation of 9α,11β‐PGF<jats:sub>2</jats:sub> to PGD<jats:sub>2</jats:sub>. The <jats:italic>k</jats:italic> <jats:sub>cat</jats:sub>/<jats:italic>K</jats:italic> <jats:sub>m</jats:sub> values for PGD<jats:sub>2</jats:sub> and 9α,11β‐PGF<jats:sub>2</jats:sub> were 21 000 and 1800 min<jats:sup>−1</jats:sup> mM<jats:sup>−1</jats:sup>, respectively, indicating that the catalytic efficiency for PGD<jats:sub>2</jats:sub> and 9α,11β‐PGF<jats:sub>2</jats:sub> was the highest among the various substrates, except for PQ. The PGFS activity in the cytosol of human lung was completely absorbed with anti‐human PGFS antiserum. Moreover, mRNA of PGFS was expressed in peripheral blood lymphocytes and the expression in lymphocytes was markedly suppressed by the T cell mitogen concanavalin A. These results support the notion that human PGFS plays an important role in the pathogenesis of allergic diseases such as asthma.</jats:p>

収録刊行物

  • FEBS Letters

    FEBS Letters 462 (3), 335-340, 1999-11-30

    Wiley

被引用文献 (16)*注記

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