Genes That Are Uniquely Stress Regulated in Salt Overly Sensitive (<i>sos</i>) Mutants

DOI PDF 1 Citations
  • Zhizhong Gong
    Center for Plant Environmental Stress Physiology, 1165 Horticulture Building, Purdue University, West Lafayette, Indiana 47907–1165 (Z.G., H.K., T.K.M., J.Z., R.A.B., P.M.H.);
  • Hisashi Koiwa
    Center for Plant Environmental Stress Physiology, 1165 Horticulture Building, Purdue University, West Lafayette, Indiana 47907–1165 (Z.G., H.K., T.K.M., J.Z., R.A.B., P.M.H.);
  • Mary Ann Cushman
    Department of Biochemistry MS200, University of Nevada, Reno, Nevada 89557–0014 (M.A.C., S.K., J.C.C.); and
  • Anamika Ray
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma 74078 (A.R., D.B.)
  • Davi Bufford
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma 74078 (A.R., D.B.)
  • Shin Kore-eda
    Department of Biochemistry MS200, University of Nevada, Reno, Nevada 89557–0014 (M.A.C., S.K., J.C.C.); and
  • Tracie K. Matsumoto
    Center for Plant Environmental Stress Physiology, 1165 Horticulture Building, Purdue University, West Lafayette, Indiana 47907–1165 (Z.G., H.K., T.K.M., J.Z., R.A.B., P.M.H.);
  • Jianhua Zhu
    Center for Plant Environmental Stress Physiology, 1165 Horticulture Building, Purdue University, West Lafayette, Indiana 47907–1165 (Z.G., H.K., T.K.M., J.Z., R.A.B., P.M.H.);
  • John C. Cushman
    Department of Biochemistry MS200, University of Nevada, Reno, Nevada 89557–0014 (M.A.C., S.K., J.C.C.); and
  • Ray A. Bressan
    Center for Plant Environmental Stress Physiology, 1165 Horticulture Building, Purdue University, West Lafayette, Indiana 47907–1165 (Z.G., H.K., T.K.M., J.Z., R.A.B., P.M.H.);
  • Paul M. Hasegawa
    Center for Plant Environmental Stress Physiology, 1165 Horticulture Building, Purdue University, West Lafayette, Indiana 47907–1165 (Z.G., H.K., T.K.M., J.Z., R.A.B., P.M.H.);

Description

<jats:title>Abstract</jats:title> <jats:p>Repetitive rounds of differential subtraction screening, followed by nucleotide sequence determination and northern-blot analysis, identified 84 salt-regulated (160 mm NaCl for 4 h) genes in Arabidopsis wild-type (Col-0 gl1) seedlings. Probes corresponding to these 84 genes and ACP1,RD22BP1, MYB2, STZ, andPAL were included in an analysis of salt responsive gene expression profiles in gl1 and the salt-hypersensitive mutant sos3. Six of 89 genes were expressed differentially in wild-type and sos3 seedlings; steady-state mRNA abundance of five genes (AD06C08/unknown, AD05E05/vegetative storage protein 2 [VSP2],AD05B11/S-adenosyl-l-Met:salicylic acid carboxyl methyltransferase [SAMT],AD03D05/cold regulated 6.6/inducible2 [COR6.6/KIN2], and salt tolerance zinc finger [STZ]) was induced and the abundance of one gene (AD05C10/circadian rhythm-RNA binding1 [CCR1]) was reduced in wild-type plants after salt treatment. The expression of CCR1, SAMT,COR6.6/KIN2, and STZ was higher insos3 than in wild type, and VSP2 andAD06C08/unknown was lower in the mutant. Salt-induced expression of VSP2 in sos1 was similar to wild type, and AD06C08/unknown, CCR1,SAMT, COR6.6/KIN2, and STZwere similar to sos3. VSP2 is regulated presumably bySOS2/3 independent of SOS1, whereas the expression of the others is SOS1 dependent.AD06C08/unknown and VSP2 are postulated to be effectors of salt tolerance whereas CCR1,SAMT, COR6.6/KIN2, and STZare determinants that must be negatively regulated during salt adaptation. The pivotal function of the SOS signal pathway to mediate ion homeostasis and salt tolerance implicatesAD06C08/unknown, VSP2,SAMT, 6.6/KIN2, STZ, andCCR1 as determinates that are involved in salt adaptation.</jats:p>

Journal

  • Plant Physiology

    Plant Physiology 126 (1), 363-375, 2001-05-01

    Oxford University Press (OUP)

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