Comparative Genomics of BCG Vaccines by Whole-Genome DNA Microarray

DOI Web Site 被引用文献30件
  • M. A. Behr
    Division of Infectious Diseases, Department of Medicine, McGill University Health Centre, Montreal H3G 1A4, Canada.
  • M. A. Wilson
    Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University, Stanford, CA 94305, USA.
  • W. P. Gill
    Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University, Stanford, CA 94305, USA.
  • H. Salamon
    Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University, Stanford, CA 94305, USA.
  • G. K. Schoolnik
    Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University, Stanford, CA 94305, USA.
  • S. Rane
    Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University, Stanford, CA 94305, USA.
  • P. M. Small
    Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University, Stanford, CA 94305, USA.

書誌事項

公開日
1999-05-28
DOI
  • 10.1126/science.284.5419.1520
公開者
American Association for the Advancement of Science (AAAS)

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説明

<jats:p> Bacille Calmette-Guérin (BCG) vaccines are live attenuated strains of <jats:italic>Mycobacterium bovis</jats:italic> administered to prevent tuberculosis. To better understand the differences between <jats:italic>M. tuberculosis</jats:italic> , <jats:italic>M. bovis</jats:italic> , and the various BCG daughter strains, their genomic compositions were studied by performing comparative hybridization experiments on a DNA microarray. Regions deleted from BCG vaccines relative to the virulent <jats:italic>M. tuberculosis</jats:italic> H37Rv reference strain were confirmed by sequencing across the missing segment of the H37Rv genome. Eleven regions (encompassing 91 open reading frames) of H37Rv were found that were absent from one or more virulent strains of <jats:italic>M. bovis</jats:italic> . Five additional regions representing 38 open reading frames were present in <jats:italic>M. bovis</jats:italic> but absent from some or all BCG strains; this is evidence for the ongoing evolution of BCG strains since their original derivation. A precise understanding of the genetic differences between closely related Mycobacteria suggests rational approaches to the design of improved diagnostics and vaccines. </jats:p>

収録刊行物

  • Science

    Science 284 (5419), 1520-1523, 1999-05-28

    American Association for the Advancement of Science (AAAS)

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