Rhizobial and Mycorrhizal Symbioses in <i>Lotus japonicus</i> Require Lectin Nucleotide Phosphohydrolase, Which Acts Upstream of Calcium Signaling
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- Nicholas J. Roberts
- Department of Molecular and Cellular Biology, University of California, Davis, California 95616 (N.J.R., G.K., A.R., M.E.E.)
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- Giulia Morieri
- John Innes Centre, Norwich NR4 7UH, United Kingdom (G.M., A.E., F.X., G.E.D.O., J.A.D.)
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- Gurpreet Kalsi
- Department of Molecular and Cellular Biology, University of California, Davis, California 95616 (N.J.R., G.K., A.R., M.E.E.)
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- Alan Rose
- Department of Molecular and Cellular Biology, University of California, Davis, California 95616 (N.J.R., G.K., A.R., M.E.E.)
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- Jiri Stiller
- Australian Research Council Centre of Excellence for Integrative Legume Research, University of Queensland, Brisbane, Queensland 4072, Australia (J.S., P.M.G.)
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- Anne Edwards
- John Innes Centre, Norwich NR4 7UH, United Kingdom (G.M., A.E., F.X., G.E.D.O., J.A.D.)
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- Fang Xie
- John Innes Centre, Norwich NR4 7UH, United Kingdom (G.M., A.E., F.X., G.E.D.O., J.A.D.)
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- Peter M. Gresshoff
- Australian Research Council Centre of Excellence for Integrative Legume Research, University of Queensland, Brisbane, Queensland 4072, Australia (J.S., P.M.G.)
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- Giles E.D. Oldroyd
- John Innes Centre, Norwich NR4 7UH, United Kingdom (G.M., A.E., F.X., G.E.D.O., J.A.D.)
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- J. Allan Downie
- John Innes Centre, Norwich NR4 7UH, United Kingdom (G.M., A.E., F.X., G.E.D.O., J.A.D.)
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- Marilynn E. Etzler
- Department of Molecular and Cellular Biology, University of California, Davis, California 95616 (N.J.R., G.K., A.R., M.E.E.)
抄録
<jats:title>Abstract</jats:title> <jats:p>Nodulation in legumes requires the recognition of rhizobially made Nod factors. Genetic studies have revealed that the perception of Nod factors involves LysM domain receptor-like kinases, while biochemical approaches have identified LECTIN NUCLEOTIDE PHOSPHOHYDROLASE (LNP) as a Nod factor-binding protein. Here, we show that antisense inhibition of LNP blocks nodulation in Lotus japonicus. This absence of nodulation was due to a defect in Nod factor signaling based on the observations that the early nodulation gene NODULE INCEPTION was not induced and that both Nod factor-induced perinuclear calcium spiking and calcium influx at the root hair tip were blocked. However, Nod factor did induce root hair deformation in the LNP antisense lines. LNP is also required for infection by the mycorrhizal fungus Glomus intraradices, suggesting that LNP plays a role in the common signaling pathway shared by the rhizobial and mycorrhizal symbioses. Taken together, these observations indicate that LNP acts at a novel position in the early stages of symbiosis signaling. We propose that LNP functions at the earliest stage of the common nodulation and mycorrhization symbiosis signaling pathway downstream of the Nod factor receptors; it may act either by influencing signaling via changes in external nucleotides or in conjunction with the LysM receptor-like kinases for recognition of Nod factor.</jats:p>
収録刊行物
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- Plant Physiology
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Plant Physiology 161 (1), 556-567, 2012-11-07
Oxford University Press (OUP)