Targeting of mTOR catalytic site inhibits multiple steps of the HIV-1 lifecycle and suppresses HIV-1 viremia in humanized mice

  • Alonso Heredia
    Institute of Human Virology, University of Maryland School of Medicine, Baltimore, MD 21201;
  • Nhut Le
    Institute of Human Virology, University of Maryland School of Medicine, Baltimore, MD 21201;
  • Ronald B. Gartenhaus
    Marlene and Stewart Greenebaum Cancer Center, University of Maryland School of Medicine, Baltimore, MD 21201
  • Edward Sausville
    Marlene and Stewart Greenebaum Cancer Center, University of Maryland School of Medicine, Baltimore, MD 21201
  • Sandra Medina-Moreno
    Institute of Human Virology, University of Maryland School of Medicine, Baltimore, MD 21201;
  • Juan C. Zapata
    Institute of Human Virology, University of Maryland School of Medicine, Baltimore, MD 21201;
  • Charles Davis
    Institute of Human Virology, University of Maryland School of Medicine, Baltimore, MD 21201;
  • Robert C. Gallo
    Institute of Human Virology, University of Maryland School of Medicine, Baltimore, MD 21201;
  • Robert R. Redfield
    Institute of Human Virology, University of Maryland School of Medicine, Baltimore, MD 21201;

書誌事項

公開日
2015-07-13
DOI
  • 10.1073/pnas.1511144112
公開者
Proceedings of the National Academy of Sciences

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説明

<jats:title>Significance</jats:title> <jats:p>Most HIV antiretrovirals target viral proteins. Unfortunately, HIV mutates under drug pressure, which can lead to drug resistance. Targeting cellular proteins that HIV necessitates in its lifecycle may help overcome HIV drug resistance because cellular proteins have lower mutations rates than do HIV proteins. Mammalian target of rapamycin (mTOR) is a cellular kinase that forms two complexes (mTORC-1 and -2), regulating protein translation and transduction signaling. We demonstrate that dual targeting of mTORC-1/2 with the catalytic inhibitor INK128 blocks HIV by interfering with entry and with transcription (basal and induced). Importantly, INK128 suppressed HIV in a preclinical animal model, suggesting that mTORC-1/2 catalytic inhibitors may help control HIV in patients, particularly in those with drug-resistant HIV.</jats:p>

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