Podocyte as the Target for Aldosterone

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  • Shigeru Shibata
    From the Department of Nephrology and Endocrinology (S.S., M.N., S.Y., T.F.), University of Tokyo Graduate School of Medicine, Bunkyo-ku, Tokyo, Japan; and the Department of Cell Biology (H.K.), Institute of Nephrology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
  • Miki Nagase
    From the Department of Nephrology and Endocrinology (S.S., M.N., S.Y., T.F.), University of Tokyo Graduate School of Medicine, Bunkyo-ku, Tokyo, Japan; and the Department of Cell Biology (H.K.), Institute of Nephrology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
  • Shigetaka Yoshida
    From the Department of Nephrology and Endocrinology (S.S., M.N., S.Y., T.F.), University of Tokyo Graduate School of Medicine, Bunkyo-ku, Tokyo, Japan; and the Department of Cell Biology (H.K.), Institute of Nephrology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
  • Hiroshi Kawachi
    From the Department of Nephrology and Endocrinology (S.S., M.N., S.Y., T.F.), University of Tokyo Graduate School of Medicine, Bunkyo-ku, Tokyo, Japan; and the Department of Cell Biology (H.K.), Institute of Nephrology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
  • Toshiro Fujita
    From the Department of Nephrology and Endocrinology (S.S., M.N., S.Y., T.F.), University of Tokyo Graduate School of Medicine, Bunkyo-ku, Tokyo, Japan; and the Department of Cell Biology (H.K.), Institute of Nephrology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.

書誌事項

タイトル別名
  • Roles of Oxidative Stress and Sgk1
公開日
2007-02
資源種別
journal article
DOI
  • 10.1161/01.hyp.0000255636.11931.a2
公開者
Ovid Technologies (Wolters Kluwer Health)

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説明

<jats:p>Accumulating evidence suggests that mineralocorticoid receptor blockade effectively reduces proteinuria in hypertensive patients. However, the mechanism of the antiproteinuric effect remains elusive. In this study, we investigated the effects of aldosterone on podocyte, a key player of the glomerular filtration barrier. Uninephrectomized rats were continuously infused with aldosterone and fed a high-salt diet. Aldosterone induced proteinuria progressively, associated with blood pressure elevation. Notably, gene expressions of podocyte-associated molecules nephrin and podocin were markedly decreased in aldosterone-infused rats at 2 weeks, with a gradual decrease thereafter. Immunohistochemical studies and electron microscopy confirmed the podocyte damage. Podocyte injury was accompanied by renal reduced nicotinamide-adenine dinucleotide phosphate oxidase activation, increased oxidative stress, and enhanced expression of aldosterone effector kinase Sgk1. Treatment with eplerenone, a selective aldosterone receptor blocker, almost completely prevented podocyte damage and proteinuria, with normalization of elevated reduced nicotinamide-adenine dinucleotide phosphate oxidase activity. In addition, proteinuria, podocyte damage, and Sgk1 upregulation were significantly alleviated by tempol, a membrane-permeable superoxide dismutase, suggesting the pathogenic role of oxidative stress. Although hydralazine treatment almost normalized blood pressure, it failed to improve proteinuria and podocyte damage. In cultured podocytes with consistent expression of mineralocorticoid receptor, aldosterone stimulated membrane translocation of reduced nicotinamide-adenine dinucleotide phosphate oxidase cytosolic components and oxidative stress generation in podocytes. Furthermore, aldosterone enhanced the expression of Sgk1, which was inhibited by mineralocorticoid receptor antagonist and tempol. In conclusion, podocytes are injured at the early stage in aldosterone-infused rats, resulting in the occurrence of proteinuria. Aldosterone can directly modulate podocyte function, possibly through the induction of oxidative stress and Sgk1.</jats:p>

収録刊行物

  • Hypertension

    Hypertension 49 (2), 355-364, 2007-02

    Ovid Technologies (Wolters Kluwer Health)

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