Mutations Affecting the Repressibility of Arginine Biosynthetic Enzymes in <i>Sacchromyces cerevisiae</i>

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<jats:p>A method is described for isolating mutants of <jats:italic>Saccharomyces cerevisiae</jats:italic> which have lost repressibility by exogenous arginine for ornithine transcarbamylase.</jats:p><jats:p>Besides permeability mutants, three complementary classes of mutations were found: argRI, argRII and argRIII which are recessive and define three loci. No evidence for a linkage between any of these three loci or with the gene coding for ornithine transcarbamylase has been obtained.</jats:p><jats:p>Strains bearing mutations at either of these loci cannot be distinguished on a phenotype basis: after growth on minimal medium, the <jats:sc>l</jats:sc>‐ornithine carbamoyl transferase activity is twice that of the wild type strain; the mutations modify neither the growth rate nor the permeability to arginine.</jats:p><jats:p>The mutations might affect the structure of an hetero‐polypeptidic aporepressor.</jats:p><jats:p>The level of ornithine transcarbamylase in diploids is proportional to the number of argF<jats:sup>+</jats:sup> genes in regulated as well as in non‐regulated cells.</jats:p>

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