Resolution of the insect ouabain paradox

  • Leah S. Torrie
    Division of Molecular Genetics, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G11 6NU, United Kingdom; and Syngenta, Alderley Park, Macclesfield, Cheshire SK10 4TF, United Kingdom
  • Jonathan C. Radford
    Division of Molecular Genetics, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G11 6NU, United Kingdom; and Syngenta, Alderley Park, Macclesfield, Cheshire SK10 4TF, United Kingdom
  • Tony D. Southall
    Division of Molecular Genetics, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G11 6NU, United Kingdom; and Syngenta, Alderley Park, Macclesfield, Cheshire SK10 4TF, United Kingdom
  • Laura Kean
    Division of Molecular Genetics, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G11 6NU, United Kingdom; and Syngenta, Alderley Park, Macclesfield, Cheshire SK10 4TF, United Kingdom
  • Andrew J. Dinsmore
    Division of Molecular Genetics, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G11 6NU, United Kingdom; and Syngenta, Alderley Park, Macclesfield, Cheshire SK10 4TF, United Kingdom
  • Shireen A. Davies
    Division of Molecular Genetics, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G11 6NU, United Kingdom; and Syngenta, Alderley Park, Macclesfield, Cheshire SK10 4TF, United Kingdom
  • Julian A. T. Dow
    Division of Molecular Genetics, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G11 6NU, United Kingdom; and Syngenta, Alderley Park, Macclesfield, Cheshire SK10 4TF, United Kingdom

書誌事項

公開日
2004-09-03
DOI
  • 10.1073/pnas.0403087101
公開者
Proceedings of the National Academy of Sciences

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説明

<jats:p> Many insects are highly resistant to plant toxins, such as the cardiac glycoside ouabain. How can the epithelia that must handle such toxins, also be refractory to them? In <jats:italic>Drosophila</jats:italic> , the Malpighian (renal) tubule contains large amounts of Na <jats:sup>+</jats:sup> ,K <jats:sup>+</jats:sup> ATPase that is known biochemically to be exquisitely sensitive to ouabain, yet the intact tissue is almost unaffected by even extraordinary concentrations. The explanation is that the tubules are protected by an active ouabain transport system, colocated with the Na <jats:sup>+</jats:sup> ,K <jats:sup>+</jats:sup> ATPase, thus preventing ouabain from reaching inhibitory concentrations within the basolateral infoldings of principal cells. These data show that the Na <jats:sup>+</jats:sup> ,K <jats:sup>+</jats:sup> ATPase, previously thought to be unimportant, may be as vital in insect tissues as in vertebrates, but can be cryptic to conventional pharmacology. </jats:p>

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