The expression of mRNA of proinflammatory cytokines during skin wound healing in mice: a preliminary study for forensic wound age estimation (II)
書誌事項
- 公開日
- 2000-05-09
- 権利情報
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- http://www.springer.com/tdm
- DOI
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- 10.1007/s004140050285
- 公開者
- Springer Science and Business Media LLC
この論文をさがす
説明
This study examined the temporal expression of mRNA for the proinflammatory cytokines, interleukin (IL)-1alpha, IL-1beta, IL-6 and tumor necrosis factor-alpha (TNFalpha) in incised wounds in mice using the reverse transcriptase-polymerase chain reaction (RT-PCR) and in situ hybridization (ISH) techniques. After incision, an increase in each cytokine mRNA level was observed by RT-PCR. The local IL-6 mRNA level peaked at 6 h, while the peak levels of mRNA for IL-1alpha, IL-1beta and TNFalpha occurred between 48 and 72 h. All cytokine mRNA levels were almost normalized after 240 h. In the early phase of wound healing, infiltrating polymorphonuclear cells were labeled with antisense probes for IL-1alpha, IL-1beta and TNFalpha mRNA by the ISH technique. Thereafter, infiltrating mononuclear cells and spindle-shaped mesenchymal cells showed positive signals for all the cytokines examined. Regenerating epidermal cells were also labeled with the antisense probes for IL-1alpha, IL-6 and TNFalpha mRNA, indicating that IL-1, IL-6, and TNFalpha are involved in skin wound healing and their local production by various cells involved in the healing process is suggested. From the viewpoint of forensic pathology, the temporal characteristics of the cytokine mRNA expression may have a potential to indicate wound age or wound vitality.
収録刊行物
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- International Journal of Legal Medicine
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International Journal of Legal Medicine 113 (3), 140-145, 2000-05-09
Springer Science and Business Media LLC
