Antioxidant Activity and Constituents of Propolis Collected in Various Areas of Korea

  • Mok-Ryeon Ahn
    Laboratory of Functional Food Science and COE Program in the 21st Century, School of Food and Nutritional Sciences, University of Shizuoka, 52-1 Yada, Shizuoka 422-8526, Japan, and Department of Food Science, Dong-A University, 840 Hadan 2-dong, Saha-gu, Busan 604-714, Korea
  • Shigenori Kumazawa
    Laboratory of Functional Food Science and COE Program in the 21st Century, School of Food and Nutritional Sciences, University of Shizuoka, 52-1 Yada, Shizuoka 422-8526, Japan, and Department of Food Science, Dong-A University, 840 Hadan 2-dong, Saha-gu, Busan 604-714, Korea
  • Tomoko Hamasaka
    Laboratory of Functional Food Science and COE Program in the 21st Century, School of Food and Nutritional Sciences, University of Shizuoka, 52-1 Yada, Shizuoka 422-8526, Japan, and Department of Food Science, Dong-A University, 840 Hadan 2-dong, Saha-gu, Busan 604-714, Korea
  • Keuk-Seung Bang
    Laboratory of Functional Food Science and COE Program in the 21st Century, School of Food and Nutritional Sciences, University of Shizuoka, 52-1 Yada, Shizuoka 422-8526, Japan, and Department of Food Science, Dong-A University, 840 Hadan 2-dong, Saha-gu, Busan 604-714, Korea
  • Tsutomu Nakayama
    Laboratory of Functional Food Science and COE Program in the 21st Century, School of Food and Nutritional Sciences, University of Shizuoka, 52-1 Yada, Shizuoka 422-8526, Japan, and Department of Food Science, Dong-A University, 840 Hadan 2-dong, Saha-gu, Busan 604-714, Korea

書誌事項

公開日
2004-11-02
DOI
  • 10.1021/jf048726s
公開者
American Chemical Society (ACS)

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説明

Propolis is a resinous substance collected by honeybees from various plant sources. The composition of propolis depends on time, vegetation, and the area of collection. This study examined the antioxidant activity of propolis from various areas of Korea: Chilgok, Cheongju, Geochang, Muju, Pocheon, and Sangju. Ethanol extracts of propolis (EEP) were prepared and evaluated for their antioxidant activity by beta-carotene bleaching, 1,1-diphenyl-2-picrylhydrazyl free radical scavenging, and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation decolorization assays. Furthermore, the major constituents in EEP were identified by high-performance liquid chromatography analysis with a photodiode array and mass spectrometric detection, and each component was quantitatively analyzed. EEP from Cheongju and Muju had relatively strong antioxidant activity accompanied by high total polyphenol contents. Propolis from Cheongju contained large amounts of antioxidative compounds, such as caffeic acid, kaempferol, and phenethyl caffeate. On the other hand, propolis from Pocheon had compounds not seen in propolis from other areas.

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