Identifying Foxp3-expressing suppressor T cells with a bicistronic reporter

DOI Web Site 被引用文献15件
  • Yisong Y. Wan
    Section of Immunobiology and Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06520
  • Richard A. Flavell
    Section of Immunobiology and Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06520

書誌事項

公開日
2005-03-28
DOI
  • 10.1073/pnas.0501701102
公開者
Proceedings of the National Academy of Sciences

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説明

<jats:p> Regulatory T cells are critical for maintaining self-tolerance and to negatively regulate immune responses. Foxp3 is a regulatory T cell-specific transcription factor that functions as the master regulator of the development and function of regulatory T cells. Here, we report the generation of a mouse model, in which a bicistronic reporter expressing a red fluorescent protein has been knocked into the endogenous Foxp3 locus. Using this mouse model, we assessed Foxp3 expression in various lymphocyte compartments and identified previously unreported Foxp3-expressing cells. In addition, we showed that <jats:italic>de novo</jats:italic> Foxp3 expression along with suppressive function were induced by TGF-β in activated CD4 T cells <jats:italic>in vitro</jats:italic> . Finally, we demonstrated that non-Foxp3-expressing CD4 T cells could not be converted into Foxp3-expressing cells upon adoptive transfer into immunodeficient hosts. This Foxp3 bicistronic reporter knockin mouse model should greatly enhance the study of regulation and function of Foxp3-expressing regulatory T cells. </jats:p>

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