A Distinct Pathway of Cell-Mediated Apoptosis Initiated by Granulysin

  • Allan A Kaspar
    Division of Immunology and Transplantation Biology, Department of Pediatrics, Stanford University School of Medicine , Stanford, CA 94305
  • Satoshi Okada
    Division of Immunology and Transplantation Biology, Department of Pediatrics, Stanford University School of Medicine , Stanford, CA 94305
  • Jayant Kumar
    Division of Immunology and Transplantation Biology, Department of Pediatrics, Stanford University School of Medicine , Stanford, CA 94305
  • Francis R Poulain
    Department of Pediatrics, University of California , San Francisco, CA 94143
  • Katerina A Drouvalakis
    Division of Immunology and Transplantation Biology, Department of Pediatrics, Stanford University School of Medicine , Stanford, CA 94305
  • Ameeta Kelekar
    Gwen Knapp Center for Lupus and Immunology Research, Howard Hughes Medical Institute, University of Chicago , Chicago, IL 60637
  • Dennis A Hanson
    Division of Immunology and Transplantation Biology, Department of Pediatrics, Stanford University School of Medicine , Stanford, CA 94305
  • Ruth M Kluck
    Division of Cellular Immunology, La Jolla Institute for Allergy and Immunology , San Diego, CA 92121
  • Yasumichi Hitoshi
    Department of Molecular Pharmacology, Stanford University School of Medicine , Stanford, CA 94305
  • Daniel E Johnson
    Department of Medicine, University of Pittsburgh , Pittsburgh, PA 15213
  • Christopher J Froelich
    Evanston Northwestern Research Institute, Northwestern University , Evanston, IL 60201
  • Craig B Thompson
    Abramson Family Cancer Research Institute, University of Pennsylvania , Philadelphia, PA 19104
  • Donald D Newmeyer
    Division of Cellular Immunology, La Jolla Institute for Allergy and Immunology , San Diego, CA 92121
  • Alberto Anel
    Department of Bioquimica, Facultad de Ciencias, Universidad de Zaragoza , Zaragoza ,
  • Carol Clayberger
    Division of Immunology and Transplantation Biology, Department of Pediatrics, Stanford University School of Medicine , Stanford, CA 94305
  • Alan M Krensky
    Division of Immunology and Transplantation Biology, Department of Pediatrics, Stanford University School of Medicine , Stanford, CA 94305

書誌事項

公開日
2001-07-01
権利情報
  • https://academic.oup.com/pages/standard-publication-reuse-rights
DOI
  • 10.4049/jimmunol.167.1.350
公開者
Oxford University Press (OUP)

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説明

<jats:title>Abstract</jats:title> <jats:p>Granulysin is an antimicrobial and tumoricidal molecule expressed in granules of CTL and NK cells. In this study, we show that granulysin damages cell membranes based upon negative charge, disrupts the transmembrane potential (Δψ) in mitochondria, and causes release of cytochrome c. Granulysin-induced apoptosis is blocked in cells overexpressing Bcl-2. Despite the release of cytochrome c, procaspase 9 is not processed. Nevertheless, activation of caspase 3 is observed in granulysin-treated cells, suggesting that granulysin activates a novel pathway of CTL- and NK cell-mediated death distinct from granzyme- and death receptor-induced apoptosis.</jats:p>

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