Newcastle Disease Virus V Protein Is Associated with Viral Pathogenesis and Functions as an Alpha Interferon Antagonist

DOI Web Site 被引用文献7件
  • Zhuhui Huang
    Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, Maryland 20742
  • Sateesh Krishnamurthy
    Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, Maryland 20742
  • Aruna Panda
    Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, Maryland 20742
  • Siba K. Samal
    Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, Maryland 20742

書誌事項

公開日
2003-08-15
権利情報
  • https://journals.asm.org/non-commercial-tdm-license
DOI
  • 10.1128/jvi.77.16.8676-8685.2003
公開者
American Society for Microbiology

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<jats:title>ABSTRACT</jats:title> <jats:p>Newcastle disease virus (NDV) edits its P gene by inserting one or two G residues at the conserved editing site (UUUUUCCC, genome sense) and transcribes the P mRNA (unedited), the V mRNA (with a +1 frameshift), and the W mRNA (with a +2 frameshift). All three proteins are amino coterminal but vary at their carboxyl terminus in length and amino acid composition. Little is known about the role of the V and W proteins in NDV replication and pathogenesis. We have constructed and recovered two recombinant viruses in which the expression of the V or both the V and W proteins has been abolished. Compared to the parental virus, the mutant viruses showed impaired growth in cell cultures, except in Vero cells. However, transient expression of the carboxyl-terminal portion of the V protein enhanced the growth of the mutant viruses. In embryonated chicken eggs, the parental virus grew to high titers in embryos of different gestational ages, whereas the mutant viruses showed an age-dependent phenomenon, growing to lower titer in more-developed embryos. An interferon (IFN) sensitivity assay showed that the parental virus was more resistant to the antiviral effect of IFN than the mutant viruses. Moreover, infection with the parental virus resulted in STAT1 protein degradation, but not with the mutant viruses. These findings indicate that the V protein of NDV possesses the ability to inhibit alpha IFN and that the IFN inhibitory function lies in the carboxyl-terminal domain. Pathogenicity studies showed that the V protein of NDV significantly contributes to the virus virulence.</jats:p>

収録刊行物

  • Journal of Virology

    Journal of Virology 77 (16), 8676-8685, 2003-08-15

    American Society for Microbiology

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