Metals and DNA: Molecular Left-Handed Complements

DOI Web Site 被引用文献26件

書誌事項

公開日
1986-08-15
DOI
  • 10.1126/science.3016894
公開者
American Association for the Advancement of Science (AAAS)

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説明

<jats:p>Chiral metal complexes provide unique molecular probes for DNA. Chiral reagents that "recognize" different local structures along the DNA strand have been designed by a process in which the asymmetry in shape and size of the complex is matched to that of the DNA helical groove. As a result, the chiral metal complexes provide very sensitive probes for local helical structure, both left- and right-handed. Direct coordination of chiral complexes to the DNA bases adds an element of sequence selectivity to the probe design. With a suitable reactive metal center, reagents that target chemically specific sites along the strand may be developed. One such chiral reagent, which cleaves left-handed DNA sites with photoactivation, has been useful in mapping this distinct conformation and examining its biological role. The conformation-specific molecular cleaver, much like a DNA-binding enzyme, recognizes and reacts at discrete sites along the DNA strand. These site-specific chiral metal complexes provide exciting new tools for probing the local variations in DNA structure and its role in the regulation of gene expression.</jats:p>

収録刊行物

  • Science

    Science 233 (4765), 727-734, 1986-08-15

    American Association for the Advancement of Science (AAAS)

被引用文献 (26)*注記

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