Microfluidic wet spinning of chitosan-alginate microfibers and encapsulation of HepG2 cells in fibers
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- Bo Ram Lee
- Korea University 1 Department of Biomedical Engineering, College of Health Science, , Seoul 136-703, South Korea
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- Kwang Ho Lee
- Korea University 1 Department of Biomedical Engineering, College of Health Science, , Seoul 136-703, South Korea
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- Edward Kang
- Korea University 1 Department of Biomedical Engineering, College of Health Science, , Seoul 136-703, South Korea
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- Dong-Sik Kim
- Korea University 3 Department of Surgery, College of Medicine, , Seoul 136-703, South Korea
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- Sang-Hoon Lee
- Korea University 1 Department of Biomedical Engineering, College of Health Science, , Seoul 136-703, South Korea
書誌事項
- 公開日
- 2011-06-01
- DOI
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- 10.1063/1.3576903
- 公開者
- AIP Publishing
説明
<jats:p>The successful encapsulation of human hepatocellular carcinoma (HepG2) cells would greatly assist a broad range of applications in tissue engineering. Due to the harsh conditions during standard chitosan fiber fabrication processes, encapsulation of HepG2 cells in chitosan fibers has been challenging. Here, we describe the successful wet-spinning of chitosan-alginate fibers using a coaxial flow microfluidic chip. We determined the optimal mixing conditions for generating chitosan-alginate fibers, including a 1:5 ratio of 2% (w/w) water-soluble chitosan (WSC) solution to 2% (w/w) alginate solution. Ratio including higher than 2% (w/w) WSC solution increased aggregation throughout the mixture. By suspending cells in the WSC–alginate solution, we successfully fabricated HepG2 cell-laden fibers. The encapsulated HepG2 cells in the chitosan-alginate fibers were more viable than cells encapsulated in pure alginate fibers, suggesting that cross-linked chitosan provides a better environment for HepG2 cells than alginate alone. In addition, we found that the adhesion of HepG2 cells on the chitosan-alginate fiber is much better than that on the alginate fibers.</jats:p>
収録刊行物
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- Biomicrofluidics
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Biomicrofluidics 5 (2), 022208-, 2011-06-01
AIP Publishing
