Nondestructive nanostraw intracellular sampling for longitudinal cell monitoring
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- Yuhong Cao
- Department of Materials Science and Engineering, Stanford University, Stanford, CA 94305;
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- Martin Hjort
- Department of Materials Science and Engineering, Stanford University, Stanford, CA 94305;
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- Haodong Chen
- Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305;
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- Fikri Birey
- Department of Psychiatry and Behavioral Sciences, Stanford University Medical School, Stanford, CA 94305;
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- Sergio A. Leal-Ortiz
- Department of Materials Science and Engineering, Stanford University, Stanford, CA 94305;
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- Crystal M. Han
- Department of Mechanical Engineering, Stanford University, Stanford, CA 94305
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- Juan G. Santiago
- Department of Mechanical Engineering, Stanford University, Stanford, CA 94305
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- Sergiu P. Paşca
- Department of Psychiatry and Behavioral Sciences, Stanford University Medical School, Stanford, CA 94305;
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- Joseph C. Wu
- Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305;
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- Nicholas A. Melosh
- Department of Materials Science and Engineering, Stanford University, Stanford, CA 94305;
書誌事項
- 公開日
- 2017-02-21
- 権利情報
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- http://www.pnas.org/site/misc/userlicense.xhtml
- DOI
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- 10.1073/pnas.1615375114
- 公開者
- Proceedings of the National Academy of Sciences
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説明
<jats:title>Significance</jats:title> <jats:p>Cell content analysis has rapidly become one of the most important new tools for measuring cell phenotype and behavior. However, the central limitation of current sampling technologies is they are destructive and must lyse the cells to measure the contents. This destruction prevents knowledge of prior or future states of the cell, which is particularly important for dynamic cell processes, such as development and differentiation. Here, we show a nondestructive longitudinal sampling and analysis platform that can sample repeatedly and accurately from the same single cell or group of cells over a long time period. We demonstrate sampling of both proteins and mRNA for cell lines as well as human-derived cardiomyocytes and astrocytes.</jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 114 (10), E1866-, 2017-02-21
Proceedings of the National Academy of Sciences
