An improved technique for isolating codominant compound microsatellite markers

DOI PDF Web Site Web Site PubMed 被引用文献36件

書誌事項

公開日
2006-04-25
権利情報
  • http://www.springer.com/tdm
DOI
  • 10.1007/s10265-006-0274-2
公開者
Springer Science and Business Media LLC

この論文をさがす

説明

An approach for developing codominant polymorphic markers (compound microsatellite (SSR) markers), with substantial time and cost savings, is introduced in this paper. In this technique, fragments flanked by a compound SSR sequence at one end were amplified from the constructed DNA library using compound SSR primer (AC)6(AG)5 or (TC)6(AC)5 and an adaptor primer for the suppression-PCR. A locus-specific primer was designed from the sequence flanking the compound SSR. The primer pairs of the locus-specific and compound SSR primers were used as a compound SSR marker. Because only one locus-specific primer was needed for design of each marker and only a common compound SSR primer was needed as the fluorescence-labeled primer for analyzing all the compound SSR markers, this approach substantially reduced the cost of developing codominant markers and analyzing their polymorphism. We have demonstrated this technique for Dendropanax trifidus and easily developed 11 codominant markers with high polymorphism for D. trifidus. Use of the technique for successful isolation of codominant compound SSR markers for several other plant species is currently in progress.

収録刊行物

被引用文献 (36)*注記

もっと見る

キーワード

問題の指摘

ページトップへ