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- Reshma Maredia
- Department of Biology, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA
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- Navya Devineni
- Department of Biology, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA
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- Peter Lentz
- Department of Biology, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA
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- Shatha F. Dallo
- Department of Biology, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA
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- JiehJuen Yu
- Department of Biology, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA
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- Neal Guentzel
- Department of Biology, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA
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- James Chambers
- Department of Biology, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA
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- Bernard Arulanandam
- Department of Biology, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA
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- William E. Haskins
- Pediatric Biochemistry Laboratory, The University of Texas at San Antonio, San Antonio, TX 78249, USA
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- Tao Weitao
- Department of Biology, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA
書誌事項
- 公開日
- 2012
- 権利情報
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- http://creativecommons.org/licenses/by/3.0/
- DOI
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- 10.1100/2012/402919
- 公開者
- Wiley
説明
<jats:p>Bacterial infections can be aggravated by antibiotic treatment that induces SOS response and vesiculation. This leads to a hypothesis concerning association of SOS with vesiculation. To test it, we conducted multiple analyses of outer membrane vesicles (OMVs) produced from the<jats:italic>Pseudomonas aeruginosa</jats:italic>wild type in which SOS is induced by ciprofloxacin and from the LexA noncleavable (<jats:italic>lexAN</jats:italic>) strain in which SOS is repressed. The levels of OMV proteins, lipids, and cytotoxicity increased for both the treated strains, demonstrating vesiculation stimulation by the antibiotic treatment. However, the further increase was suppressed in the<jats:italic>lexAN</jats:italic>strains, suggesting the SOS involvement. Obviously, the stimulated vesiculation is attributed by both SOS-related and unrelated factors. OMV subproteomic analysis was performed to examine these factors, which reflected the OMV-mediated cytotoxicity and the physiology of the vesiculating cells under treatment and SOS. Thus, SOS plays a role in the vesiculation stimulation that contributes to cytotoxicity.</jats:p>
収録刊行物
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- The Scientific World Journal
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The Scientific World Journal 2012 1-18, 2012
Wiley