THE BIOSYNTHESIS OF ERYTHRITOL AND GLYCEROL BY TORULOPSIS MAGNOLIAE. STUDIES WITH C<sup>14</sup>-LABELLED GLUCOSE

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<jats:p>D-Glucose-1-C<jats:sup>14</jats:sup>, D-glucose-2-C<jats:sup>14</jats:sup>, D-glucose-3, 4-C<jats:sup>14</jats:sup>, and D-glucose-6-C<jats:sup>14</jats:sup>were dissimilated aerobically by washed cells of Torulopsis magnoliae. The major products formed were erythritol and glycerol.A method for the stepwise degradation of erythritol, after oxidation with Acetobacter suboxydans to L-erythrulose, is described. The distribution of radioactive carbon in the erythritol suggests that it is formed by a complex series of reactions in which enzymes of the transaldolase and transketolase type play a major part to give a four-carbon fragment which is then reduced to the tetritol. Enzymes of the Embden–Meyerhof pathway are probably concerned to a lesser extent.</jats:p>

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