Rice WRKY45 Plays a Crucial Role in Benzothiadiazole-Inducible Blast Resistance

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  • Masaki Shimono
    Plant Disease Resistance Research Unit, National Institute of Agrobiological Sciences, Ibaraki 305-8602, Japan
  • Shoji Sugano
    Plant Disease Resistance Research Unit, National Institute of Agrobiological Sciences, Ibaraki 305-8602, Japan
  • Akira Nakayama
    Plant Disease Resistance Research Unit, National Institute of Agrobiological Sciences, Ibaraki 305-8602, Japan
  • Chang-Jie Jiang
    Plant Disease Resistance Research Unit, National Institute of Agrobiological Sciences, Ibaraki 305-8602, Japan
  • Kazuko Ono
    Plant Genetic Engineering Research Unit, National Institute of Agrobiological Sciences, Ibaraki 305-8602, Japan
  • Seiichi Toki
    Plant Genetic Engineering Research Unit, National Institute of Agrobiological Sciences, Ibaraki 305-8602, Japan
  • Hiroshi Takatsuji
    Plant Disease Resistance Research Unit, National Institute of Agrobiological Sciences, Ibaraki 305-8602, Japan

書誌事項

公開日
2007-06-01
権利情報
  • http://creativecommons.org/licenses/by-nc/4.0/
DOI
  • 10.1105/tpc.106.046250
公開者
Oxford University Press (OUP)

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説明

<jats:title>Abstract</jats:title><jats:p>Benzothiadiazole (BTH) is a so-called plant activator and protects plants from diseases by activating the salicylic acid (SA) signaling pathway. By microarray screening, we identified BTH- and SA-inducible WRKY transcription factor (TF) genes that were upregulated within 3 h after BTH treatment. Overexpression of one of them, WRKY45, in rice (Oryza sativa) markedly enhanced resistance to rice blast fungus. RNA interference–mediated knockdown of WRKY45 compromised BTH-inducible resistance to blast disease, indicating that it is essential for BTH-induced defense responses. In a transient expression system, WRKY45 activated reporter gene transcription through W-boxes. Epistasis analysis suggested that WRKY45 acts in the SA signaling pathway independently of NH1, a rice ortholog of Arabidopsis thaliana NPR1, which distinguishes WRKY45 from known Arabidopsis WRKY TFs. Two defense-related genes, encoding a glutathione S-transferase and a cytochrome P450, were found to be regulated downstream of WRKY45 but were not regulated by NH1, consistent with the apparent independence of the WRKY45- and NH1-dependent pathways. Defense gene expression in WRKY45-overexpressed rice plants varied with growth conditions, suggesting that some environmental factor(s) acts downstream of WRKY45 transcription. We propose a role for WRKY45 in BTH-induced and SA-mediated defense signaling in rice and its potential utility in improving disease resistance of rice, an importance food resource worldwide.</jats:p>

収録刊行物

  • The Plant Cell

    The Plant Cell 19 (6), 2064-2076, 2007-06-01

    Oxford University Press (OUP)

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