The Arabidopsis Botrytis Susceptible1 Interactor Defines a Subclass of RING E3 Ligases That Regulate Pathogen and Stress Responses
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- Hongli Luo
- Department of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana 47907–2054 (H.L., K.L., Z.L., T.M.); Department of Plant Pathology, North Carolina State University, Raleigh, North Carolina 27606 (P.V.); Department of Plant Protection, Zhejiang University-Huajiachi Campus, Hangzhou, Zhejiang 310029, People’s Republic of China (F.S.)
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- Kristin Laluk
- Department of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana 47907–2054 (H.L., K.L., Z.L., T.M.); Department of Plant Pathology, North Carolina State University, Raleigh, North Carolina 27606 (P.V.); Department of Plant Protection, Zhejiang University-Huajiachi Campus, Hangzhou, Zhejiang 310029, People’s Republic of China (F.S.)
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- Zhibing Lai
- Department of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana 47907–2054 (H.L., K.L., Z.L., T.M.); Department of Plant Pathology, North Carolina State University, Raleigh, North Carolina 27606 (P.V.); Department of Plant Protection, Zhejiang University-Huajiachi Campus, Hangzhou, Zhejiang 310029, People’s Republic of China (F.S.)
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- Paola Veronese
- Department of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana 47907–2054 (H.L., K.L., Z.L., T.M.); Department of Plant Pathology, North Carolina State University, Raleigh, North Carolina 27606 (P.V.); Department of Plant Protection, Zhejiang University-Huajiachi Campus, Hangzhou, Zhejiang 310029, People’s Republic of China (F.S.)
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- Fengming Song
- Department of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana 47907–2054 (H.L., K.L., Z.L., T.M.); Department of Plant Pathology, North Carolina State University, Raleigh, North Carolina 27606 (P.V.); Department of Plant Protection, Zhejiang University-Huajiachi Campus, Hangzhou, Zhejiang 310029, People’s Republic of China (F.S.)
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- Tesfaye Mengiste
- Department of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana 47907–2054 (H.L., K.L., Z.L., T.M.); Department of Plant Pathology, North Carolina State University, Raleigh, North Carolina 27606 (P.V.); Department of Plant Protection, Zhejiang University-Huajiachi Campus, Hangzhou, Zhejiang 310029, People’s Republic of China (F.S.)
抄録
<jats:title>Abstract</jats:title> <jats:p>We studied the function of Arabidopsis (Arabidopsis thaliana) Botrytis Susceptible1 Interactor (BOI) in plant responses to pathogen infection and abiotic stress. BOI physically interacts with and ubiquitinates Arabidopsis BOS1, an R2R3MYB transcription factor previously implicated in stress and pathogen responses. In transgenic plants expressing the BOS1-β-glucuronidase transgene, β-glucuronidase activity could be detected only after inhibition of the proteosome, suggesting that BOS1 is a target of ubiquitin-mediated degradation by the proteosome. Plants with reduced BOI transcript levels generated through RNA interference (BOI RNAi) were more susceptible to the necrotrophic fungus Botrytis cinerea and less tolerant to salt stress. In addition, BOI RNAi plants exhibited increased cell death induced by the phytotoxin α-picolinic acid and by a virulent strain of the bacterial pathogen Pseudomonas syringae, coincident with peak disease symptoms. However, the hypersensitive cell death associated with different race-specific resistance genes was unaffected by changes in the level of BOI transcript. BOI expression was enhanced by B. cinerea and salt stress but repressed by the plant hormone gibberellin, indicating a complex regulation of BOI gene expression. Interestingly, BOI RNAi plants exhibit reduced growth responsiveness to gibberellin. We also present data revealing the function of three Arabidopsis BOI-RELATED GENES (BRGs), which contribute to B. cinerea resistance and the suppression of disease-associated cell death. In sum, BOI and BRGs represent a subclass of RING E3 ligases that contribute to plant disease resistance and abiotic stress tolerance through the suppression of pathogen-induced as well as stress-induced cell death.</jats:p>
収録刊行物
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- Plant Physiology
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Plant Physiology 154 (4), 1766-1782, 2010-10-04
Oxford University Press (OUP)