<i>In vitro</i> self-assembly of tailorable nanotubes from a simple protein building block
-
- Edward R. Ballister
- *Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA 94720;
-
- Angela H. Lai
- W. M. Keck Advanced Microscopy Laboratory, Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158; and
-
- Ronald N. Zuckermann
- *Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA 94720;
-
- Yifan Cheng
- W. M. Keck Advanced Microscopy Laboratory, Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158; and
-
- Joseph D. Mougous
- Department of Microbiology, University of Washington, Seattle, WA 98195
書誌事項
- 公開日
- 2008-03-11
- DOI
-
- 10.1073/pnas.0712247105
- 公開者
- Proceedings of the National Academy of Sciences
この論文をさがす
説明
<jats:p> We demonstrate a method for generating discretely structured protein nanotubes from the simple ring-shaped building block, homohexameric Hcp1 from <jats:italic>Pseudomonas aeruginosa</jats:italic> . Our design exploited the observation that the crystal lattice of Hcp1 contains rings stacked in a repeating head-to-tail pattern. High-resolution detail of the ring–ring interface allowed the selection of sites for specific cysteine mutations capable of engaging in disulfide bond formation across rings, thereby generating stable Hcp1 nanotubes. Protein nanotubes containing up to 25 subunits (≈100 nm in length) were self-assembled under simple conditions. Furthermore, we demonstrate that the tube ends and interior can be independently and specifically functionalized to generate nanocapsules. </jats:p>
収録刊行物
-
- Proceedings of the National Academy of Sciences
-
Proceedings of the National Academy of Sciences 105 (10), 3733-3738, 2008-03-11
Proceedings of the National Academy of Sciences