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- Cuicui Ge
- Chinese Academy of Sciences Key Lab for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100190, China;
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- Jiangfeng Du
- Chinese Academy of Sciences Key Lab for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100190, China;
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- Lina Zhao
- Chinese Academy of Sciences Key Lab for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100190, China;
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- Liming Wang
- Chinese Academy of Sciences Key Lab for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100190, China;
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- Ying Liu
- Chinese Academy of Sciences Key Lab for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100190, China;
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- Denghua Li
- Chinese Academy of Sciences Key Lab for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100190, China;
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- Yanlian Yang
- Chinese Academy of Sciences Key Lab for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100190, China;
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- Ruhong Zhou
- Computational Biology Center, IBM Thomas J. Watson Research Center, Yorktown Heights, NY 10598;
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- Yuliang Zhao
- Chinese Academy of Sciences Key Lab for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100190, China;
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- Zhifang Chai
- Chinese Academy of Sciences Key Lab for Nuclear Analytical Techniques, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China
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- Chunying Chen
- Chinese Academy of Sciences Key Lab for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100190, China;
書誌事項
- 公開日
- 2011-10-03
- DOI
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- 10.1073/pnas.1105270108
- 公開者
- Proceedings of the National Academy of Sciences
この論文をさがす
説明
<jats:p> With the potential wide uses of nanoparticles such as carbon nanotubes in biomedical applications, and the growing concerns of nanotoxicity of these engineered nanoparticles, the importance of nanoparticle–protein interactions cannot be stressed enough. In this study, we use both experimental and theoretical approaches, including atomic force microscope images, fluorescence spectroscopy, CD, SDS-PAGE, and molecular dynamics simulations, to investigate the interactions of single-wall carbon nanotubes (SWCNTs) with human serum proteins, and find a competitive binding of these proteins with different adsorption capacity and packing modes. The <jats:italic>π</jats:italic> - <jats:italic>π</jats:italic> stacking interactions between SWCNTs and aromatic residues (Trp, Phe, Tyr) are found to play a critical role in determining their adsorption capacity. Additional cellular cytotoxicity assays, with human acute monocytic leukemia cell line and human umbilical vein endothelial cells, reveal that the competitive bindings of blood proteins on the SWCNT surface can greatly alter their cellular interaction pathways and result in much reduced cytotoxicity for these protein-coated SWCNTs, according to their respective adsorption capacity. These findings have shed light toward the design of safe carbon nanotube nanomaterials by comprehensive preconsideration of their interactions with human serum proteins. </jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 108 (41), 16968-16973, 2011-10-03
Proceedings of the National Academy of Sciences