Receptor and effector interactions of G<sub>s</sub> Functional studies with antibodies to the α<sub>s</sub> carboxyl‐terminal decapeptide
書誌事項
- 公開日
- 1989-06-05
- 権利情報
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- http://onlinelibrary.wiley.com/termsAndConditions#vor
- DOI
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- 10.1016/0014-5793(89)80622-2
- 公開者
- Wiley
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説明
<jats:p>Antibodies generated to a synthetic decapeptide, RMHLRQYELL, representing the carboxyl‐terminus of G<jats:sub>s</jats:sub>‐α have been characterized in immunoblots and functional studies. This antibody, designated RM, reacts exclusively with a doublet of proteins of 52 and 45 kDa in immunoblots of bovine brain and wild‐type S49 murine lymphoma cell membranes. No such reactivity is seen in membranes from <jats:italic>cyc</jats:italic> <jats:sup>−</jats:sup> S49 cells, which lack G<jats:sub>s</jats:sub>. RM blocks receptor‐mediated activation of G<jats:sub>s</jats:sub> and adenylyl cyclase in membranes from wild‐type S49 cells. RM could also immunoprecipitate adenylyl cyclase activity in detergent extracts from GTP[γ]S‐ or fluoride‐preactivated bovine brain membranes; thus binding of α<jats:sub>s</jats:sub> to effector and carboxyl‐terminal antibody was mutually compatible. Such experiments provide an approach for the elucidation of functionally relevant interactions of G‐proteins with receptors and electors in the membrane.</jats:p>
収録刊行物
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- FEBS Letters
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FEBS Letters 249 (2), 189-194, 1989-06-05
Wiley