Novel Application of Ag Nanoclusters in Fluorescent Imaging of Human Serum Proteins after Native Polyacrylamide Gel Electrophoresis (PAGE)
書誌事項
- 公開日
- 2012-01-16
- 権利情報
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- http://onlinelibrary.wiley.com/termsAndConditions#vor
- DOI
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- 10.1002/chem.201101310
- 公開者
- Wiley
この論文をさがす
説明
<jats:title>Abstract</jats:title><jats:p>We have developed a novel application for DNA oligonucleotide‐stabilized Ag nanoclusters in fluorescent imaging of human serum proteins after native polyacrylamide gel electrophoresis (PAGE). Oligonucleotide‐stabilized Ag nanoclusters were used as fluorescent probes for direct detection of proteins after native PAGE. Some relatively low‐abundance proteins, such as α‐1‐antichymotrypsin (ACT) and α‐2‐glycoprotein 1, zinc (ZAG) were easily detected by oligonucleotide‐stabilized Ag nanocluster‐based fluorescent imaging and identified by MS and MS/MS techniques, without the need of expensive antibodies or tedious immunoassay procedures. The pH condition for the oligonucleotide‐stabilized Ag nanocluster solution was optimized and the possible mechanism of interaction between proteins and DNA oligonucleotide‐stabilized Ag nanoclusters was analyzed. As a novel fluorescent detection method it is simple, fast, nontoxic and sensitive, and it shows great analytical potential in proteome research and in biochemistry.</jats:p>
収録刊行物
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- Chemistry – A European Journal
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Chemistry – A European Journal 18 (5), 1432-1437, 2012-01-16
Wiley