Mobile platform for rapid sub–picogram-per-milliliter, multiplexed, digital droplet detection of proteins

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  • Venkata Yelleswarapu
    School of Engineering and Applied Science, Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104-6321;
  • Joshua R. Buser
    Chip Diagnostics, Philadelphia, PA 19104;
  • Margalit Haber
    School of Engineering and Applied Science, Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104-6321;
  • Jonathan Baron
    School of Engineering and Applied Science, Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104-6321;
  • Eshwar Inapuri
    School of Engineering and Applied Science, Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104-6321;
  • David Issadore
    School of Engineering and Applied Science, Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104-6321;

説明

<jats:title>Significance</jats:title> <jats:p> Digital assays have enormous untapped potential for diagnostics, environmental surveillance, and biosafety monitoring, but are currently confined to laboratory settings due to the instrumentation necessary to generate, control, and measure millions of droplets. We instead use a mobile phone-based imaging technique that is <jats:inline-formula> <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" overflow="scroll"> <mml:mo>></mml:mo> </mml:math> </jats:inline-formula> 100× faster than conventional microfluidic droplet detection, does not require expensive optics, is invariant to flow rate, and can simultaneously measure multiple fluorescent dyes in droplets. By using this time domain modulation with cloud computing, we overcome the low frame rate of digital imaging, and achieve throughputs as high as 1 million droplets per second. We integrate on-chip delay lines and a microbead processing unit, resulting in a robust device, suitable for low-cost implementation, with ultrasensitive measurement capabilities. </jats:p>

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