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- Kamran Ghaedi
- Department of Biology, Graduate School of Science, Kyushu University, Fukuoka 812-8581, Japan;
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- Shigehiko Tamura
- Department of Biology, Graduate School of Science, Kyushu University, Fukuoka 812-8581, Japan;
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- Kanji Okumoto
- Department of Biology, Graduate School of Science, Kyushu University, Fukuoka 812-8581, Japan;
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- Yuji Matsuzono
- Department of Biology, Graduate School of Science, Kyushu University, Fukuoka 812-8581, Japan;
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- Yukio Fujiki
- Department of Biology, Graduate School of Science, Kyushu University, Fukuoka 812-8581, Japan;
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- Guido Guidotti
- editor
抄録
<jats:p>Rat cDNA encoding a 372-amino-acid peroxin was isolated, primarily by functional complementation screening, using a peroxisome-deficient Chinese hamster ovary cell mutant, ZPG208, of complementation group 17. The deduced primary sequence showed ∼25% amino acid identity with the yeast Pex3p, thereby we termed this cDNA ratPEX3 (RnPEX3). Human and Chinese hamster Pex3p showed 96 and 94% identity to rat Pex3p and had 373 amino acids. Pex3p was characterized as an integral membrane protein of peroxisomes, exposing its N- and C-terminal parts to the cytosol. A homozygous, inactivating missense mutation, G to A at position413, in a codon (GGA) for Gly<jats:sup>138</jats:sup>and resulting in a codon (GAA) for Glu was the genetic cause of peroxisome deficiency of complementation group 17 ZPG208. The peroxisome-restoring activity apparently required the full length of Pex3p, whereas its N-terminal part from residues 1 to 40 was sufficient to target a fusion protein to peroxisomes. We also demonstrated that Pex3p binds the farnesylated peroxisomal membrane protein Pex19p. Moreover, upon expression of PEX3 in ZPG208, peroxisomal membrane vesicles were assembled before the import of soluble proteins such as PTS2-tagged green fluorescent protein. Thus, Pex3p assembles membrane vesicles before the matrix proteins are translocated.</jats:p>
収録刊行物
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- Molecular Biology of the Cell
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Molecular Biology of the Cell 11 (6), 2085-2102, 2000-06
American Society for Cell Biology (ASCB)