Determination of DNA base compositions from melting profiles in dilute buffers

DOI Web Site Web Site 被引用文献2件

書誌事項

公開日
1969-04
権利情報
  • http://onlinelibrary.wiley.com/termsAndConditions#vor
DOI
  • 10.1002/bip.1969.360070408
公開者
Wiley

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説明

<jats:title>Abstract</jats:title><jats:p>Equations were determined for the dependency of the melting temperature (<jats:italic>T</jats:italic><jats:sub>m</jats:sub>) of DNA upon the logarithm of the sodium ion concentration, for four DNA samples of widely different base compositions (θ<jats:sub>GC</jats:sub>). The slopes of these <jats:italic>T</jats:italic><jats:sub>m</jats:sub> versus log <jats:italic>M</jats:italic> equations wore found to decrease with increasing θ<jats:sub>G C</jats:sub>of the samples. An empirical equation relating <jats:italic>T</jats:italic><jats:sub>m</jats:sub>, log <jats:italic>M</jats:italic> (Na<jats:sup>+</jats:sup>) and θ<jats:sub>G C</jats:sub> was derived, which also accounts for differences in <jats:italic>T</jats:italic><jats:sub>m</jats:sub> versus log <jats:italic>M</jats:italic> slopes. Data from the literature for some synthetic polynucleotides and for the crab(<jats:italic>Cancer pagarus</jats:italic>) satellite poly AT are discussed in relation to the above finding. The changes in <jats:italic>T</jats:italic><jats:sub>m</jats:sub> versus log <jats:italic>M</jats:italic> slopes with θ<jats:sub>G C</jats:sub> are interpreted in terms of changes in the thermodynamic parameters Δ<jats:italic>S</jats:italic> and Δ<jats:italic>H</jats:italic> with base composition.</jats:p>

収録刊行物

  • Biopolymers

    Biopolymers 7 (4), 503-516, 1969-04

    Wiley

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