Alternate fast and slow stepping of a heterodimeric kinesin molecule
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説明
A conventional kinesin molecule travels continuously along a microtubule in discrete 8-nm steps. This processive movement is generally explained by models in which the two identical heads of a kinesin move in a 'hand-over-hand' manner. Here, we show that a single heterodimeric kinesin molecule (in which one of the two heads is mutated in a nucleotide-binding site) exhibits fast and slow (with the dwell time at least 10 times longer than that of the fast step) 8-nm steps alternately, presumably corresponding to the displacement by the wild-type and mutant heads, respectively. Our results provide the first direct evidence for models in which the roles of the two heads alternate every 8-nm step.
収録刊行物
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- Nature Cell Biology
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Nature Cell Biology 5 (12), 1079-1082, 2003-11-23
Springer Science and Business Media LLC
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詳細情報 詳細情報について
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- CRID
- 1362262945736307200
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- DOI
- 10.1038/ncb1067
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- ISSN
- 14764679
- 14657392
- http://id.crossref.org/issn/14764679
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- PubMed
- 14647299
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- データソース種別
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- Crossref
- OpenAIRE