High Prevalence of<i>Candida</i>Yeast in Milk Samples from Cows Suffering from Mastitis in Poland

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  • Bozena Dworecka-Kaszak
    Mycology Division, Preclinical Science Department, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Ciszewskiego 8, 02-789 Warsaw, Poland
  • Alicja Krutkiewicz
    Mycology Division, Preclinical Science Department, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Ciszewskiego 8, 02-789 Warsaw, Poland
  • Daniel Szopa
    Molecular Genetic Division, Mother and Child institute, Kasprzaka 17a, 01-211 Warsaw, Poland
  • Miroslaw Kleczkowski
    Division of Laboratory Diagnostics, Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warsaw, Poland
  • Malgorzata Biegańska
    Mycology Division, Preclinical Science Department, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Ciszewskiego 8, 02-789 Warsaw, Poland

説明

<jats:p>Mastitis is an economically important disease in which fungi belonging to the genus<jats:italic>Candida</jats:italic>may participate as etiological agents. This study focused on determining the frequency of fungal isolation and differentiation of fungal species isolated from milk of mastitic cows. Sixty-six milk samples from mastitic cows were studied, and 55 strains of fungi were isolated. Seven different species classified as<jats:italic>Candida</jats:italic>were identified basing on phenotypic properties, and the dominating species was<jats:italic>C. parapsilosis</jats:italic>. Genomic DNA was isolated and amplified in PCR with ITS1 and NL2 primers. Amplification products were digested with restriction enzymes<jats:italic>Hpa</jats:italic>II and<jats:italic>EcoR</jats:italic>I. Amplification of DNA with ITS1 and NL2 primers resulted in products of different sizes. Comparison of product sizes in restriction fragment PCR REA confirmed differences among species. Strains grouped together on the basis of phenotype characteristics differed in restriction fragment profiles. None of the investigated species showed similar genetic profiles.</jats:p>

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