Two moles of O2 consumption and one mole of H2O2 formation during cholesterol peroxidation with cholesterol oxidase from <i>Pseudomonas</i> sp. strain ST-200
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- Noriyuki DOUKYU
- Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta 4259, Midori-ku, Yokohama, 226-8051, Japan
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- Rikizo AONO
- Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta 4259, Midori-ku, Yokohama, 226-8051, Japan
書誌事項
- 公開日
- 1999-07-26
- DOI
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- 10.1042/bj3410621
- 10.1042/0264-6021:3410621
- 公開者
- Portland Press Ltd.
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説明
<jats:p>Cholesterol oxidase from Pseudomonas sp. strain ST-200 oxidized cholesterol and cholestanol to 6β-hydroperoxycholest-4-en-3-one and 5α-cholestan-3-one respectively. The former was converted spontaneously to several oxysteroids such as 6-hydroxycholest-4-en-3-one and cholest-4-ene-3,6-dione, with the consumption of 2 mol of O2 and the formation of 1 mol of H2O2 for each mole of cholesterol oxidized. An oxidized form of the cholesterol oxidase dehydrogenates cholesterol, probably to the 5-en-3-one derivative. A reduced form of the enzyme, yielded from the cholesterol dehydrogenation reaction, dioxygenated cholest-5-en-3-one to 6β-hydroperoxycholest-4-en-3-one.</jats:p>
収録刊行物
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- Biochemical Journal
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Biochemical Journal 341 (3), 621-627, 1999-07-26
Portland Press Ltd.