Simultaneous Separation, Metering, and Dilution of Plasma from Human Whole Blood in a Microfluidic System
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- Tomoya Tachi
- Department of Applied Chemistry, Graduate School of Engineering, MEXT Innovative Research Center for Preventive Medical Engineering, Plasma Nanotechnology Research Center, Nagoya University, Nagoya 464-8603, Japan, Health Technology Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Takamatsu 761-0395, Japan, and Institute for Molecular Science, National Institutes of Natural Sciences, Okazaki 444-8585, Japan
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- Noritada Kaji
- Department of Applied Chemistry, Graduate School of Engineering, MEXT Innovative Research Center for Preventive Medical Engineering, Plasma Nanotechnology Research Center, Nagoya University, Nagoya 464-8603, Japan, Health Technology Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Takamatsu 761-0395, Japan, and Institute for Molecular Science, National Institutes of Natural Sciences, Okazaki 444-8585, Japan
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- Manabu Tokeshi
- Department of Applied Chemistry, Graduate School of Engineering, MEXT Innovative Research Center for Preventive Medical Engineering, Plasma Nanotechnology Research Center, Nagoya University, Nagoya 464-8603, Japan, Health Technology Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Takamatsu 761-0395, Japan, and Institute for Molecular Science, National Institutes of Natural Sciences, Okazaki 444-8585, Japan
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- Yoshinobu Baba
- Department of Applied Chemistry, Graduate School of Engineering, MEXT Innovative Research Center for Preventive Medical Engineering, Plasma Nanotechnology Research Center, Nagoya University, Nagoya 464-8603, Japan, Health Technology Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Takamatsu 761-0395, Japan, and Institute for Molecular Science, National Institutes of Natural Sciences, Okazaki 444-8585, Japan
書誌事項
- 公開日
- 2009-03-13
- DOI
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- 10.1021/ac802434z
- 公開者
- American Chemical Society (ACS)
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説明
In about a 3 min period, we have simultaneously separated plasma from human whole blood and metered and diluted the plasma using a microchip with an interchannel microstructure. The plasma separation was based on both cross-flow filtration and sedimentation of red blood cells in the microchannels. Metering and diluting operations of the plasma were based on volume control of liquid in the microchannels by syringe pumps. On this microchip, we produced plasma diluted by a factor of 6 from whole blood containing theophylline and we observed very little hemolysis. It is possible to separate plasma from one or just several drops of whole blood by using this microchip.
収録刊行物
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- Analytical Chemistry
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Analytical Chemistry 81 (8), 3194-3198, 2009-03-13
American Chemical Society (ACS)