Isolation and Characterization of a Rice Dwarf Mutant with a Defect in Brassinosteroid Biosynthesis
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- Masaki Mori
- Departments of Molecular Genetics (M.M., H.O., Ka.S., K.O., Ko.S., H.H., S.K.) and
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- Takahito Nomura
- Department of Biosciences, Teikyo University, Utsunomiya, Tochigi 320–8551, Japan (T.N., T.Y.);
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- Hisako Ooka
- Departments of Molecular Genetics (M.M., H.O., Ka.S., K.O., Ko.S., H.H., S.K.) and
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- Masumi Ishizaka
- Chemical Analysis Research Center, National Institute for Agro-Environmental Sciences, Tsukuba, Ibaraki 305–8604, Japan (M.I.)
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- Takao Yokota
- Department of Biosciences, Teikyo University, Utsunomiya, Tochigi 320–8551, Japan (T.N., T.Y.);
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- Kazuhiko Sugimoto
- Departments of Molecular Genetics (M.M., H.O., Ka.S., K.O., Ko.S., H.H., S.K.) and
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- Ken Okabe
- Departments of Molecular Genetics (M.M., H.O., Ka.S., K.O., Ko.S., H.H., S.K.) and
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- Hideyuki Kajiwara
- Biochemistry (H.K.), National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305–8602, Japan;
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- Koji Yamamoto
- Nagaoka University of Technology, Nagaoka, Niigata 940–2137, Japan (H.O., K.Y.); and
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- Hirohiko Hirochika
- Departments of Molecular Genetics (M.M., H.O., Ka.S., K.O., Ko.S., H.H., S.K.) and
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- Shoshi Kikuchi
- Departments of Molecular Genetics (M.M., H.O., Ka.S., K.O., Ko.S., H.H., S.K.) and
書誌事項
- 公開日
- 2002-11-01
- 権利情報
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- https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model
- DOI
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- 10.1104/pp.007179
- 公開者
- Oxford University Press (OUP)
この論文をさがす
説明
<jats:title>Abstract</jats:title><jats:p>We have isolated a new recessive dwarf mutant of rice (Oryza sativa L. cv Nipponbare). Under normal growth conditions, the mutant has very short leaf sheaths; has short, curled, and frizzled leaf blades; has few tillers; and is sterile. Longitudinal sections of the leaf sheaths revealed that the cell length along the longitudinal axis is reduced, which explains the short leaf sheaths. Transverse sections of the leaf blades revealed enlargement of the motor cells along the dorsal-ventral axis, which explains the curled and frizzled leaf blades. In addition, the number of crown roots was smaller and the growth of branch roots was weaker than those in the wild-type plant. Because exogenously supplied brassinolide considerably restored the normal phenotypes, we designated the mutantbrassinosteroid-dependent 1(brd1). Further, under darkness, brd1showed constitutive photomorphogenesis. Quantitative analyses of endogenous sterols and brassinosteroids (BRs) indicated that BR-6-oxidase, a BR biosynthesis enzyme, would be defective. In fact, a 0.2-kb deletion was detected in the genomic region ofOsBR6ox (a rice BR-6-oxidase gene) in thebrd1 mutant. These results indicate that BRs are involved in many morphological and physiological processes in rice, including the elongation and unrolling of leaves, development of tillers, skotomorphogenesis, root differentiation, and reproductive growth, and that the defect of BR-6-oxidase caused thebrd1 phenotype.</jats:p>
収録刊行物
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- Plant Physiology
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Plant Physiology 130 (3), 1152-1161, 2002-11-01
Oxford University Press (OUP)
