Skin resident memory CD8+ T cells are phenotypically and functionally distinct from circulating populations and lack immediate cytotoxic function
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- J A Seidel
- Division of Infection and Immunity, University College London, UK
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- M Vukmanovic-Stejic
- Division of Infection and Immunity, University College London, UK
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- B Muller-Durovic
- Division of Infection and Immunity, University College London, UK
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- N Patel
- Division of Infection and Immunity, University College London, UK
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- J Fuentes-Duculan
- Laboratory for Investigative Dermatology, The Rockefeller University, New York, USA
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- S M Henson
- Division of Infection and Immunity, University College London, UK
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- J G Krueger
- Laboratory for Investigative Dermatology, The Rockefeller University, New York, USA
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- M H A Rustin
- Department of Dermatology, The Royal Free Hospital, London, UK
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- F O Nestle
- NIHR Biomedical Research Centre, Cutaneous Medicine and Immunotherapy, St John's Institute of Dermatology, Division of Genetics and Molecular Medicine, Guy's Hospital, King's College London, London, UK
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- K E Lacy
- NIHR Biomedical Research Centre, Cutaneous Medicine and Immunotherapy, St John's Institute of Dermatology, Division of Genetics and Molecular Medicine, Guy's Hospital, King's College London, London, UK
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- A N Akbar
- Division of Infection and Immunity, University College London, UK
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説明
<jats:title>Summary</jats:title> <jats:p>The in-depth understanding of skin resident memory CD8+ T lymphocytes (TRM) may help to uncover strategies for their manipulation during disease. We investigated isolated TRM from healthy human skin, which expressed the residence marker CD69, and compared them to circulating CD8+ T cell populations from the same donors. There were significantly increased proportions of CD8+CD45RA−CD27− T cells in the skin that expressed low levels of killer cell lectin-like receptor G1 (KLRG1), CD57, perforin and granzyme B. The CD8+ TRM in skin were therefore phenotypically distinct from circulating CD8+CD45RA−CD27− T cells that expressed high levels of all these molecules. Nevertheless, the activation of CD8+ TRM with T cell receptor (TCR)/CD28 or interleukin (IL)-2 or IL-15 in vitro induced the expression of granzyme B. Blocking signalling through the inhibitory receptor programmed cell death 1 (PD)-1 further boosted granzyme B expression. A unique feature of some CD8+ TRM cells was their ability to secrete high levels of tumour necrosis factor (TNF)-α and IL-2, a cytokine combination that was not seen frequently in circulating CD8+ T cells. The cutaneous CD8+ TRM are therefore diverse, and appear to be phenotypically and functionally distinct from circulating cells. Indeed, the surface receptors used to distinguish differentiation stages of blood T cells cannot be applied to T cells in the skin. Furthermore, the function of cutaneous TRM appears to be stringently controlled by environmental signals in situ.</jats:p>
収録刊行物
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- Clinical and Experimental Immunology
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Clinical and Experimental Immunology 194 (1), 79-92, 2018-09-12
Oxford University Press (OUP)