Significant contribution of the <i>pgdA</i> gene to the virulence of <i>Streptococcus suis</i>

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公開日
2008-11-04
権利情報
  • http://onlinelibrary.wiley.com/termsAndConditions#vor
DOI
  • 10.1111/j.1365-2958.2008.06463.x
公開者
Wiley

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説明

<jats:title>Summary</jats:title><jats:p> <jats:italic>Streptococcus suis</jats:italic> is a major swine pathogen and emerging zoonotic agent. In this study we have determined the muropeptide composition of <jats:italic>S. suis</jats:italic> peptidoglycan (PG) and found, among other modifications, N‐deacetylated compounds. Comparison with an isogenic mutant showed that the product of the <jats:italic>pgdA</jats:italic> gene is responsible for this specific modification which occurred in very low amounts. Low level of PG N‐deacetylation correlated with absence of significant lysozyme resistance when wild‐type <jats:italic>S. suis</jats:italic> was grown <jats:italic>in vitro</jats:italic>. On the other hand, expression of the <jats:italic>pgdA</jats:italic> gene was increased upon interaction of the bacterium with neutrophils <jats:italic>in vitro</jats:italic> as well as <jats:italic>in vivo</jats:italic> in experimentally inoculated mice, suggesting that <jats:italic>S. suis</jats:italic> may enhance PG N‐deacetylation under these conditions. Evaluation of the Δ<jats:italic>pgdA</jats:italic> mutant in both the CD1 murine and the porcine models of infection revealed a significant contribution of the <jats:italic>pgdA</jats:italic> gene to the virulence traits of <jats:italic>S. suis</jats:italic>. Reflecting a severe impairment in its ability to persist in blood and decreased ability to escape immune clearance mechanisms mediated by neutrophils, the Δ<jats:italic>pgdA</jats:italic> mutant was highly attenuated in both models. The results of this study suggest that modification of PG by N‐deacetylation is an important factor in <jats:italic>S. suis</jats:italic> virulence.</jats:p>

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