Gene expression profiles of epithelial cells microscopically isolated from a breast-invasive ductal carcinoma and a nodal metastasis
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- I. Zucchi
- Istituto di Tecnologie Biomediche, Consiglio Nazionale delle Ricerche, Via F. lli Cervi 93, 20090 Segrate-Milan, Italy; Genome Institute of Singapore, Department of Information and Math Sciences, 60 Biopolis Street, Singapore 138672; Centro Ricerche Interdipartimentale Biotecnologie Innovative, University of Padua, 35121 Padua, Italy; Istituto Nazionale Tumori, Via Venezian 31, 20133 Milan, Italy; Max Planck Institute for Molecular Biomedicine, Cell and Developmental Biology, D48149 Muenster,...
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- E. Mento
- Istituto di Tecnologie Biomediche, Consiglio Nazionale delle Ricerche, Via F. lli Cervi 93, 20090 Segrate-Milan, Italy; Genome Institute of Singapore, Department of Information and Math Sciences, 60 Biopolis Street, Singapore 138672; Centro Ricerche Interdipartimentale Biotecnologie Innovative, University of Padua, 35121 Padua, Italy; Istituto Nazionale Tumori, Via Venezian 31, 20133 Milan, Italy; Max Planck Institute for Molecular Biomedicine, Cell and Developmental Biology, D48149 Muenster,...
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- V. A. Kuznetsov
- Istituto di Tecnologie Biomediche, Consiglio Nazionale delle Ricerche, Via F. lli Cervi 93, 20090 Segrate-Milan, Italy; Genome Institute of Singapore, Department of Information and Math Sciences, 60 Biopolis Street, Singapore 138672; Centro Ricerche Interdipartimentale Biotecnologie Innovative, University of Padua, 35121 Padua, Italy; Istituto Nazionale Tumori, Via Venezian 31, 20133 Milan, Italy; Max Planck Institute for Molecular Biomedicine, Cell and Developmental Biology, D48149 Muenster,...
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- M. Scotti
- Istituto di Tecnologie Biomediche, Consiglio Nazionale delle Ricerche, Via F. lli Cervi 93, 20090 Segrate-Milan, Italy; Genome Institute of Singapore, Department of Information and Math Sciences, 60 Biopolis Street, Singapore 138672; Centro Ricerche Interdipartimentale Biotecnologie Innovative, University of Padua, 35121 Padua, Italy; Istituto Nazionale Tumori, Via Venezian 31, 20133 Milan, Italy; Max Planck Institute for Molecular Biomedicine, Cell and Developmental Biology, D48149 Muenster,...
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- V. Valsecchi
- Istituto di Tecnologie Biomediche, Consiglio Nazionale delle Ricerche, Via F. lli Cervi 93, 20090 Segrate-Milan, Italy; Genome Institute of Singapore, Department of Information and Math Sciences, 60 Biopolis Street, Singapore 138672; Centro Ricerche Interdipartimentale Biotecnologie Innovative, University of Padua, 35121 Padua, Italy; Istituto Nazionale Tumori, Via Venezian 31, 20133 Milan, Italy; Max Planck Institute for Molecular Biomedicine, Cell and Developmental Biology, D48149 Muenster,...
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- B. Simionati
- Istituto di Tecnologie Biomediche, Consiglio Nazionale delle Ricerche, Via F. lli Cervi 93, 20090 Segrate-Milan, Italy; Genome Institute of Singapore, Department of Information and Math Sciences, 60 Biopolis Street, Singapore 138672; Centro Ricerche Interdipartimentale Biotecnologie Innovative, University of Padua, 35121 Padua, Italy; Istituto Nazionale Tumori, Via Venezian 31, 20133 Milan, Italy; Max Planck Institute for Molecular Biomedicine, Cell and Developmental Biology, D48149 Muenster,...
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- E. Vicinanza
- Istituto di Tecnologie Biomediche, Consiglio Nazionale delle Ricerche, Via F. lli Cervi 93, 20090 Segrate-Milan, Italy; Genome Institute of Singapore, Department of Information and Math Sciences, 60 Biopolis Street, Singapore 138672; Centro Ricerche Interdipartimentale Biotecnologie Innovative, University of Padua, 35121 Padua, Italy; Istituto Nazionale Tumori, Via Venezian 31, 20133 Milan, Italy; Max Planck Institute for Molecular Biomedicine, Cell and Developmental Biology, D48149 Muenster,...
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- G. Valle
- Istituto di Tecnologie Biomediche, Consiglio Nazionale delle Ricerche, Via F. lli Cervi 93, 20090 Segrate-Milan, Italy; Genome Institute of Singapore, Department of Information and Math Sciences, 60 Biopolis Street, Singapore 138672; Centro Ricerche Interdipartimentale Biotecnologie Innovative, University of Padua, 35121 Padua, Italy; Istituto Nazionale Tumori, Via Venezian 31, 20133 Milan, Italy; Max Planck Institute for Molecular Biomedicine, Cell and Developmental Biology, D48149 Muenster,...
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- S. Pilotti
- Istituto di Tecnologie Biomediche, Consiglio Nazionale delle Ricerche, Via F. lli Cervi 93, 20090 Segrate-Milan, Italy; Genome Institute of Singapore, Department of Information and Math Sciences, 60 Biopolis Street, Singapore 138672; Centro Ricerche Interdipartimentale Biotecnologie Innovative, University of Padua, 35121 Padua, Italy; Istituto Nazionale Tumori, Via Venezian 31, 20133 Milan, Italy; Max Planck Institute for Molecular Biomedicine, Cell and Developmental Biology, D48149 Muenster,...
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- R. Reinbold
- Istituto di Tecnologie Biomediche, Consiglio Nazionale delle Ricerche, Via F. lli Cervi 93, 20090 Segrate-Milan, Italy; Genome Institute of Singapore, Department of Information and Math Sciences, 60 Biopolis Street, Singapore 138672; Centro Ricerche Interdipartimentale Biotecnologie Innovative, University of Padua, 35121 Padua, Italy; Istituto Nazionale Tumori, Via Venezian 31, 20133 Milan, Italy; Max Planck Institute for Molecular Biomedicine, Cell and Developmental Biology, D48149 Muenster,...
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- P. Vezzoni
- Istituto di Tecnologie Biomediche, Consiglio Nazionale delle Ricerche, Via F. lli Cervi 93, 20090 Segrate-Milan, Italy; Genome Institute of Singapore, Department of Information and Math Sciences, 60 Biopolis Street, Singapore 138672; Centro Ricerche Interdipartimentale Biotecnologie Innovative, University of Padua, 35121 Padua, Italy; Istituto Nazionale Tumori, Via Venezian 31, 20133 Milan, Italy; Max Planck Institute for Molecular Biomedicine, Cell and Developmental Biology, D48149 Muenster,...
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- A. Albertini
- Istituto di Tecnologie Biomediche, Consiglio Nazionale delle Ricerche, Via F. lli Cervi 93, 20090 Segrate-Milan, Italy; Genome Institute of Singapore, Department of Information and Math Sciences, 60 Biopolis Street, Singapore 138672; Centro Ricerche Interdipartimentale Biotecnologie Innovative, University of Padua, 35121 Padua, Italy; Istituto Nazionale Tumori, Via Venezian 31, 20133 Milan, Italy; Max Planck Institute for Molecular Biomedicine, Cell and Developmental Biology, D48149 Muenster,...
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- R. Dulbecco
- Istituto di Tecnologie Biomediche, Consiglio Nazionale delle Ricerche, Via F. lli Cervi 93, 20090 Segrate-Milan, Italy; Genome Institute of Singapore, Department of Information and Math Sciences, 60 Biopolis Street, Singapore 138672; Centro Ricerche Interdipartimentale Biotecnologie Innovative, University of Padua, 35121 Padua, Italy; Istituto Nazionale Tumori, Via Venezian 31, 20133 Milan, Italy; Max Planck Institute for Molecular Biomedicine, Cell and Developmental Biology, D48149 Muenster,...
説明
<jats:p> Expression profiles of breast carcinomas are difficult to interpret when they are obtained from tissue <jats:italic>in toto</jats:italic> , which may contain a large proportion of non-cancer cells. To avoid this problem, we microscopically isolated cells from a primary invasive ductal carcinoma of the breast and from an axillary node harboring a metastatic breast carcinoma, to obtain pure populations of carcinoma cells (≈500) and used them for serial analysis of gene expression. The expression profiles generated from both populations of cells were compared with the profile of a disease-free mammary epithelium. We showed that the expression profiles obtained are exclusive of carcinoma cells with no contribution of non-epithelial cells. From a total of 16,939 unique tags analyzed, we detected 559 statistically significant changes in gene expression; some of these genes have not been previously associated with breast cancer. We observed that many of the down-regulated genes are the same in both cancers, whereas the up-regulated genes are completely different, suggesting that the down-regulation of a set of genes may be the basic mechanism of cancer formation, while the up-regulation may characterize and possibly control the state of evolution of individual cancers. The results obtained may help in characterizing the neoplastic process of breast cancer. </jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 101 (52), 18147-18152, 2004-12-17
Proceedings of the National Academy of Sciences