Regulation of meiotic prophase arrest in mouse oocytes by GPR3, a constitutive activator of the Gs G protein
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- Leon Freudzon
- 1Department of Cell Biology, University of Connecticut Health Center, Farmington, CT 06032
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- Rachael P. Norris
- 1Department of Cell Biology, University of Connecticut Health Center, Farmington, CT 06032
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- Arthur R. Hand
- 2Department of Pediatric Dentistry, University of Connecticut Health Center, Farmington, CT 06032
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- Shigeru Tanaka
- 3Department of Neurological Surgery, The Ohio State University, Columbus, OH 43210
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- Yoshinaga Saeki
- 3Department of Neurological Surgery, The Ohio State University, Columbus, OH 43210
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- Teresa L.Z. Jones
- 4National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892
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- Mark M. Rasenick
- 5Department of Physiology and Biophysics, University of Illinois at Chicago, Chicago, IL 60612
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- Catherine H. Berlot
- 7Weis Center for Research, Geisinger Clinic, Danville, PA 17822
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- Lisa M. Mehlmann
- 1Department of Cell Biology, University of Connecticut Health Center, Farmington, CT 06032
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- Laurinda A. Jaffe
- 1Department of Cell Biology, University of Connecticut Health Center, Farmington, CT 06032
説明
<jats:p>The arrest of meiotic prophase in mouse oocytes within antral follicles requires the G protein Gs and an orphan member of the G protein–coupled receptor family, GPR3. To determine whether GPR3 activates Gs, the localization of Gαs in follicle-enclosed oocytes from Gpr3+/+ and Gpr3−/− mice was compared by using immunofluorescence and GαsGFP. GPR3 decreased the ratio of Gαs in the oocyte plasma membrane versus the cytoplasm and also decreased the amount of Gαs in the oocyte. Both of these properties indicate that GPR3 activates Gs. The follicle cells around the oocyte are also necessary to keep the oocyte in prophase, suggesting that they might activate GPR3. However, GPR3-dependent Gs activity was similar in follicle-enclosed and follicle-free oocytes. Thus, the maintenance of prophase arrest depends on the constitutive activity of GPR3 in the oocyte, and the follicle cell signal acts by a means other than increasing GPR3 activity.</jats:p>
収録刊行物
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- The Journal of Cell Biology
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The Journal of Cell Biology 171 (2), 255-265, 2005-10-24
Rockefeller University Press